| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCC11 |
| Uniprot No | Q96J66 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCC11重组蛋白的3篇参考文献示例(注：部分内容为假设性示例，实际引用时请核实文献真实性)：
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1. **文献名称**：*Functional Characterization of Recombinant ABCC11 Expressed in Mammalian Cells*
**作者**：Smith J, et al.
**摘要**：本研究在HEK293细胞中成功表达并纯化ABCC11重组蛋白，利用膜囊泡转运实验证实其可主动转运环状核苷酸(如cGMP)和类固醇代谢物(如硫酸雌酮)。实验发现，ABCC11的转运活性依赖ATP水解，并可被MK571等抑制剂阻断，提示其在药物外排和代谢中的潜在作用。
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2. **文献名称**：*Cryo-EM Structure of Human ABCC11 Reveals Substrate-binding Pocket Dynamics*
**作者**：Tanaka K, et al.
**摘要**：通过冷冻电镜解析ABCC11重组蛋白的3.8 Å分辨率结构，揭示其跨膜结构域和核苷酸结合域(NBD)的构象变化。研究发现了底物结合口袋的关键残基，并通过突变实验证明Gly180Arg突变会破坏底物识别，为腋臭相关突变的功能缺失机制提供结构依据。
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3. **文献名称**：*ABCC11 Mutants Impair Recombinant Protein Stability and Lipid Transport in Axillary Osmidrosis*
**作者**：Chen L, et al.
**摘要**：分析ABCC11常见突变体(如rs17822931)对重组蛋白功能的影响。结果显示，突变导致蛋白稳定性下降及短链脂肪酸转运能力丧失，解释其与腋臭表型的关联，并提示ABCC11在皮肤脂质分泌中的关键作用。
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4. **文献名称**：*High-throughput Screening of ABCC11 ATPase Activity Using Purified Recombinant Protein*
**作者**：Müller R, et al.
**摘要**：开发了一种基于重组ABCC11蛋白的ATP酶活性检测方法，用于高通量筛选调节剂。研究发现，特定底物(如cGMP)可显著增强ATP水解活性，为靶向ABCC11的药物开发提供工具。
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**注意**：以上文献为示例性质，实际研究中请通过PubMed或Web of Science等数据库检索真实文献(关键词：ABCC11 recombinant, ABCC11 expression, ABCC11 structure)。真实文献可能涉及其在乳腺癌耐药性、耳垢表型或代谢疾病中的研究。
**Background of ABCC11 Recombinant Protein**
ABCC11 (ATP-binding cassette subfamily C member 11), also known as MRP8. is a transmembrane protein belonging to the ABC transporter superfamily, which plays critical roles in ATP-dependent transport of various substrates across cellular membranes. This protein is primarily expressed in tissues such as apocrine glands, breast, and earwax-producing cells. ABCC11 is notably associated with the secretion of physiological metabolites, including steroids, cyclic nucleotides, and odorants, and has been implicated in drug resistance mechanisms by effluxing therapeutic agents.
The ABCC11 gene exhibits a clinically significant single-nucleotide polymorphism (SNP, rs17822931) that determines earwax type (wet or dry) and axillary odor. The non-functional allele (homozygous recessive) leads to dry earwax and reduced body odor, a trait prevalent in East Asian populations. This polymorphism has made ABCC11 a subject of interest in population genetics and personal care product development.
Recombinant ABCC11 protein is produced using expression systems like mammalian cells or insect cells to ensure proper post-translational modifications and membrane localization. It is purified via affinity chromatography, often tagged with His or FLAG for detection. Studies leveraging recombinant ABCC11 aim to elucidate its substrate specificity, transport mechanisms, and interactions with inhibitors. Its role in drug resistance and metabolic disorders (e.g., hyperuricemia) has spurred research into therapeutic targeting. Additionally, recombinant ABCC11 is used in cosmetic science to develop inhibitors targeting body odor pathways.
Overall, ABCC11 recombinant protein serves as a vital tool for dissecting its biological functions and exploring clinical applications in precision medicine and biotechnology.
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