| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC69 |
| Uniprot No | A6NI79 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-296aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMGCRHSR LSSCKPPKKK RQEPEPEQPP RPEPHELGPL NGDTAITVQL CASEEAERHQ KDITRILQQH EEEKKKWAQQ VEKERELELR DRLDEQQRVL EGKNEEALQV LRASYEQEKE ALTHSFREAS STQQETIDRL TSQLEAFQAK MKRVEESILS RNYKKHIQDY GSPSQFWEQE LESLHFVIEM KNERIHELDR RLILMETVKE KNLILEEKIT TLQQENEDLH VRSRNQVVLS RQLSEDLLLT REALEKEVQL RRQLQQEKEE LLYRVLGANA SPAFPLAPVT PTEVSFLAT |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCDC69重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Structural and functional characterization of CCDC69 as a regulator of centriole duplication*
**作者**：Smith A, et al.
**摘要**：本研究利用重组CCDC69蛋白解析其晶体结构，发现其通过结合CEP250调控中心体复制。实验表明，CCDC69缺失会导致中心体异常分裂，揭示其在细胞周期中的关键作用。
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2. **文献名称**：*CCDC69 interacts with CEP250 to maintain centrosome cohesion and ciliogenesis*
**作者**：Lee J, Kim H.
**摘要**：通过重组CCDC69蛋白的体外结合实验，证明其与CEP250直接相互作用，维持中心体粘附及纤毛形成。敲低CCDC69的细胞模型显示纤毛生成缺陷，提示其在发育疾病中的潜在影响。
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3. **文献名称**：*Recombinant CCDC69 reveals a role in DNA damage response via Aurora kinase signaling*
**作者**：Wang Y, et al.
**摘要**：研究利用重组CCDC69蛋白进行互作组分析，发现其与Aurora激酶通路相关蛋白结合，参与DNA损伤修复。功能实验表明CCDC69缺失导致染色体不稳定，可能关联癌症发生机制。
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(注：以上文献为示例，实际文献需根据具体数据库检索结果补充。)
CCDC69 (Coiled-Coil Domain-Containing Protein 69) is a relatively understudied protein implicated in various cellular processes, particularly those involving structural organization and regulatory functions. It belongs to the family of coiled-coil domain-containing proteins, which are characterized by their alpha-helical structures that mediate protein-protein interactions. CCDC69 is encoded by the *CCDC69* gene, located on human chromosome 12. and is evolutionarily conserved across vertebrates, suggesting its fundamental biological role.
Research indicates that CCDC69 is involved in mitotic cell division, where it interacts with components of the chromosomal passenger complex (CPC) and the kinetochore, playing a role in ensuring proper chromosome segregation. It has also been associated with primary cilia formation, a critical structure for cellular signaling and sensory functions. Dysregulation of CCDC69 has been linked to genomic instability and may contribute to cancer progression, though mechanistic details remain unclear.
Recombinant CCDC69 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cell lines) to enable functional studies. Its recombinant form allows researchers to investigate binding partners, structural motifs, and post-translational modifications. Purification often involves affinity tags (e.g., His-tag) followed by chromatographic techniques. Studies using recombinant CCDC69 have facilitated the identification of its interaction with Aurora B kinase and survivin, highlighting its potential role in mitotic regulation.
Despite emerging interest, the full scope of CCDC69’s functions and regulatory mechanisms remains to be elucidated. Ongoing research aims to clarify its involvement in diseases, particularly cancers, and explore its utility as a therapeutic target or diagnostic marker.
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