| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GFI1B |
| Uniprot No | Q5VTD9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-330aa |
| 氨基酸序列 | MPRSFLVKSKKAHTYHQPRVQEDEPLWPPALTPVPRDQAPSNSPVLSTLFPNQCLDWTNLKREPELEQDQNLARMAPAPEGPIVLSRPQDGDSPLSDSPPFYKPSFSWDTLATTYGHSYRQAPSTMQSAFLEHSVSLYGSPLVPSTEPALDFSLRYSPGMDAYHCVKCNKVFSTPHGLEVHVRRSHSGTRPFACDICGKTFGHAVSLEQHTHVHSQERSFECRMCGKAFKRSSTLSTHLLIHSDTRPYPCQFCGKRFHQKSDMKKHTYIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFSCELCTKGFQRKVDLRRHRESQHNLK |
| 分子量 | 63.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人GFI1B蛋白的关键参考文献(人工模拟数据，仅供格式参考)：
1. **文献名称**: "GFI1B controls human erythroid and megakaryocytic differentiation by regulating TGF-β signaling"
**作者**: Sankaran VG, et al.
**摘要**: 揭示了重组人GFI1B蛋白通过调控TGF-β通路在红系和巨核细胞分化中的关键作用，并利用体外重组蛋白实验证明其与DNA结合的分子机制。
2. **文献名称**: "Structural basis of GFI1B-mediated transcriptional repression in hematopoiesis"
**作者**: Zhou Y, et al.
**摘要**: 通过X射线晶体学解析重组人GFI1B蛋白的锌指结构域三维结构，阐明其与靶基因启动子结合的分子基础及表观遗传调控机制。
3. **文献名称**: "GFI1B mutation causes a novel bleeding disorder with macrothrombocytopenia"
**作者**: Monteferrario D, et al.
**摘要**: 利用重组GFI1B蛋白功能实验证实其突变体导致巨血小板减少症的病理机制，提出靶向修复策略的治疗潜力。
注：以上为模拟案例，真实文献需通过PubMed或Google Scholar检索关键词"recombinant human GFI1B"、"GFI1B protein function"等获取。
**Background of Recombinant Human GFI1B Protein**
The Growth Factor Independent 1B (GFI1B) protein is a zinc finger transcriptional repressor critical in hematopoiesis and cellular differentiation. It belongs to the GFI family, which includes GFI1. sharing structural homology but distinct regulatory roles. GFI1B is essential for megakaryocyte development, erythroid maturation, and maintenance of hematopoietic stem cells. It functions by binding DNA via its C-terminal zinc finger domains and recruiting chromatin-modifying complexes, such as histone deacetylases (HDACs) or methyltransferases, to suppress target gene expression.
Mutations in the *GFI1B* gene are linked to blood disorders, including thrombocytopenia, anemia, and myelodysplasia, underscoring its role in blood cell homeostasis. Recombinant human GFI1B protein, produced via expression systems like *E. coli* or mammalian cells, retains these functional domains, enabling *in vitro* studies to dissect its molecular mechanisms. Researchers use it to explore interactions with co-repressors, DNA-binding specificity, and regulatory networks in normal and malignant hematopoiesis. Additionally, it serves as a tool for drug discovery targeting GFI1B-related pathologies.
The protein’s application extends to developmental biology, particularly in modeling blood diseases and stem cell differentiation. Its recombinant form ensures controlled, high-purity studies, bridging gaps between genetic insights and therapeutic interventions.
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