| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GABRG1 |
| Uniprot No | Q8N1C3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-465aa |
| 氨基酸序列 | MGPLKAFLFSPFLLRSQSRGVRLVFLLLTLHLGNCVDKADDEDDEDLTVNKTWVLAPKIHEGDITQILNSLLQGYDNKLRPDIGVRPTVIETDVYVNSIGPVDPINMEYTIDIIFAQTWFDSRLKFNSTMKVLMLNSNMVGKIWIPDTFFRNSRKSDAHWITTPNRLLRIWNDGRVLYTLRLTINAECYLQLHNFPMDEHSCPLEFSSYGYPKNEIEYKWKKPSVEVADPKYWRLYQFAFVGLRNSTEITHTISGDYVIMTIFFDLSRRMGYFTIQTYIPCILTVVLSWVSFWINKDAVPARTSLGITTVLTMTTLSTIARKSLPKVSYVTAMDLFVSVCFIFVFAALMEYGTLHYFTSNQKGKTATKDRKLKNKASMTPGLHPGSTLIPMNNISVPQEDDYEYQCLEGKDCASFFCCFEDCRTGSWREGRIHIRIAKIDSYSRIFFPTAFALFNLVYWVGYLYL |
| 分子量 | 80.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人GABRG1蛋白的相关文献摘要：
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1. **文献名称**: "Expression and functional characterization of recombinant human GABA_A receptor γ1 subunit"
**作者**: Siegwart R et al.
**摘要**: 该研究成功在哺乳动物细胞系中表达了重组人GABRG1蛋白，发现γ1亚基与α/β亚基共表达时形成功能性GABA_A受体，并展现出独特的苯二氮䓬类药物结合特性，提示γ1亚基可能影响受体药理学特性。
2. **文献名称**: "Assembly and trafficking of GABA_A receptor γ1 subunit-containing receptors"
**作者**: Connolly CN et al.
**摘要**: 通过重组表达系统探究GABRG1亚基在受体组装和细胞膜运输中的作用，发现γ1与γ2亚基存在差异，可能导致特定脑区受体亚型的特异性分布及突触功能调节。
3. **文献名称**: "Structural insights into the human GABA_A receptor γ1 subunit by cryo-EM"
**作者**: Kim JJ, Möhler H.
**摘要**: 利用冷冻电镜解析了含GABRG1亚基的受体复合物结构，揭示其跨膜结构域的关键氨基酸残基对离子通道门控和配体结合的作用，为靶向γ1亚基的药物设计提供依据。
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注：上述文献为假设性示例，实际研究中建议通过PubMed或Google Scholar以关键词“recombinant human GABRG1”“GABRG1 protein functional analysis”等检索最新论文。
Recombinant human GABRG1 protein is a genetically engineered form of the γ1 subunit of the gamma-aminobutyric acid type A (GABAₐ) receptor, a critical ligand-gated ion channel in the central nervous system. GABAₐ receptors mediate fast inhibitory neurotransmission by facilitating chloride ion influx upon GABA binding, thereby regulating neuronal excitability. The γ1 subunit (encoded by the *GABRG1* gene) contributes to receptor assembly, synaptic localization, and modulation of pharmacological properties, though it is less characterized compared to other γ subunits (e.g., γ2). Recombinant GABRG1 is typically expressed in mammalian (e.g., HEK293) or insect cell systems (e.g., Sf9) to ensure proper post-translational modifications, such as glycosylation, crucial for structural and functional integrity. Its production enables detailed studies on GABAₐ receptor composition, gating mechanisms, and interactions with therapeutics like benzodiazepines. Research utilizing recombinant GABRG1 focuses on elucidating its role in receptor function, pathogenic mutations linked to epilepsy or neuropsychiatric disorders, and structure-based drug design. This tool also aids in reconstituting functional receptors in heterologous systems, advancing insights into subunit-specific contributions to inhibitory signaling and disease pathology.
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