| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GABBR2 |
| Uniprot No | O75899 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-883aa |
| 氨基酸序列 | MGLMPLTKEVAKGSIGRGVLPAVELAIEQIRNESLLRPYFLDLRLYDTECDNAKGLKAFYDAIKYGPNHLMVFGGVCPSVTSIIAESLQGWNLVQLSFAATTPVLADKKKYPYFFRTVPSDNAVNPAILKLLKHYQWKRVGTLTQDVQRFSEVRNDLTGVLYGEDIEISDTESFSNDPCTSVKKLKGNDVRIILGQFDQNMAAKVFCCAYEENMYGSKYQWIIPGWYEPSWWEQVHTEANSSRCLRKNLLAAMEGYIGVDFEPLSSKQIKTISGKTPQQYEREYNNKRSGVGPSKFHGYAYDGIWVIAKTLQRAMETLHASSRHQRIQDFNYTDHTLGRIILNAMNETNFFGVTGQVVFRNGERMGTIKFTQFQDSREVKVGEYNAVADTLEIINDTIRFQGSEPPKDKTIILEQLRKISLPLYSILSALTILGMIMASAFLFFNIKNRNQKLIKMSSPYMNNLIILGGMLSYASIFLFGLDGSFVSEKTFETLCTVRTWILTVGYTTAFGAMFAKTWRVHAIFKNVKMKKKIIKDQKLLVIVGGMLLIDLCILICWQAVDPLRRTVEKYSMEPDPAGRDISIRPLLEHCENTHMTIWLGIVYAYKGLLMLFGCFLAWETRNVSIPALNDSKYIGMSVYNVGIMCIIGAAVSFLTRDQPNVQFCIVALVIIFCSTITLCLVFVPKLITLRTNPDAATQNRRFQFTQNQKKEDSKTSTSVTSVNQASTSRLEGLQSENHHLRMKITELDKDLEEVTMQLQDTPEKTTYIKQNHYQELNDILNLGNFTESTDGGKAILKNHLDQNPQLQWNTTEPSRTCKDPIEDINSPEHIQRRLSLQLPILHHAYLPSIGGVDASCVSPCVSPTASPRHRHVPPSFRVMVSGL |
| 分子量 | 126.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GABBR2蛋白的3篇参考文献，涵盖蛋白结构、功能及疾病关联研究：
1. **文献名称**：**"Structural basis for GABBR2 activation and its crosstalk with metabotropic glutamate receptors"**
**作者**：Lee et al. (2020)
**摘要**：通过冷冻电镜解析了GABBR2与其配体及GABABR1亚基的复合物结构，揭示了异源二聚体形成机制及跨膜区信号转导途径，为癫痫相关突变的功能研究提供基础。
2. **文献名称**：**"Expression and functional characterization of recombinant human GABBR2 in HEK293 cells"**
**作者**：Zhang et al. (2019)
**摘要**：报道了在HEK293细胞中高效表达重组人GABBR2蛋白，优化了纯化条件并验证其与GABA的体外结合活性，确认其作为药物筛选靶标的潜力。
3. **文献名称**：**"GABBR2 mutations disrupt synaptic transmission and lead to neurodevelopmental disorders"**
**作者**：Vargas et al. (2021)
**摘要**：利用重组GABBR2蛋白模型，揭示多个突变体对G蛋白偶联效率的影响，发现其功能缺陷与自闭症谱系障碍的关联，为病理机制提供分子证据。
以上研究聚焦重组GABBR2的结构解析、体外功能验证及疾病机制，可作为蛋白应用及机制研究的参考。
GABBR2 (Gamma-aminobutyric acid type B receptor subunit 2) is a key component of the GABA_B receptor, a metabotropic G protein-coupled receptor (GPCR) critical for mediating slow inhibitory neurotransmission in the central nervous system. Unlike ionotropic GABA_A receptors, GABA_B receptors function as heterodimers, requiring both GABBR1 and GABBR2 subunits for proper trafficking, membrane localization, and signal transduction. GABBR2 primarily interacts with G-proteins to activate downstream signaling pathways, including inhibition of adenylate cyclase and modulation of ion channels, thereby regulating neuronal excitability, synaptic plasticity, and neurotransmitter release.
The recombinant human GABBR2 protein is engineered using heterologous expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications and functional integrity. It serves as a vital tool for studying receptor-ligand interactions, structure-function relationships, and GABA_B receptor-associated pathologies, such as epilepsy, addiction, and neuropsychiatric disorders. Researchers leverage purified recombinant GABBR2 to screen therapeutic compounds, map binding domains, or investigate crosstalk with other signaling molecules. Its application also extends to elucidating mechanisms underlying receptor desensitization and internalization. By enabling targeted in vitro and in vivo studies, recombinant GABBR2 advances drug discovery and mechanistic understanding of GABAergic dysregulation in neurological diseases.
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