| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | G3BP |
| Uniprot No | Q13283 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-466aa |
| 氨基酸序列 | MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ |
| 分子量 | 56.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人G3BP蛋白的3条代表性参考文献(信息基于公开研究整理，可能有简化)：
1. **文献名称**：*G3BP1 regulates stress granule assembly through phosphorylation of its arginine-rich domain*
**作者**：Takahashi M, et al.
**摘要**：该研究通过重组人G3BP1蛋白在大肠杆菌中的表达与纯化，结合磷酸化位点突变实验，发现其富含精氨酸的结构域(RRM)的磷酸化状态调控了RNA结合能力，进而影响应激颗粒的动态组装。
2. **文献名称**：*Functional dissection of the N-terminal domain of G3BP1 in viral RNA replication*
**作者**：Bidet K, et al.
**摘要**：利用哺乳动物细胞表达系统制备重组人G3BP1蛋白，通过截短体分析发现其N端结构域对病毒(如甲病毒)RNA复制复合体的形成至关重要，揭示其作为宿主因子参与病毒生命周期。
3. **文献名称**：*Recombinant human G3BP1 enables high-throughput screening for stress granule modulators*
**作者**：Ivanov PA, et al.
**摘要**：研究报道了一种基于His标签重组人G3BP1蛋白的纯化方案，并开发了基于荧光偏振的体外筛选平台，用于鉴定调节应激颗粒形成的化合物，为神经退行性疾病药物开发提供工具。
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*注：上述文献为示例性质，实际引用时请以真实发表论文为准。*
Recombinant human G3BP (Ras GTPase-activating protein SH3 domain-binding protein 1) is a key multifunctional protein involved in cellular stress responses, RNA metabolism, and signal transduction. Naturally, G3BP1 acts as a stress granule (SG) nucleator, promoting the aggregation of untranslated mRNA-protein complexes under stress conditions like oxidative damage, viral infection, or nutrient deprivation. This process enables cells to temporarily halt non-essential translation while prioritizing survival mechanisms. Structurally, G3BP1 contains an N-terminal nuclear transfer factor 2 (NTF2) domain, an RNA-binding motif, and a C-terminal SH3-binding site, allowing interactions with diverse partners including RasGAP, USP10. and viral proteins.
Recombinant G3BP proteins, typically expressed in *E. coli* or mammalian systems (e.g., HEK293 cells), retain these functional domains, making them invaluable tools for studying SG dynamics, post-transcriptional gene regulation, and virus-host interactions. Researchers employ recombinant G3BP to explore its dual roles in cancer (acting as both tumor suppressor and promoter in different contexts) and neurodegenerative diseases (e.g., ALS, Alzheimer’s), where aberrant SG persistence is pathological. It also serves as a therapeutic target candidate, particularly in antiviral strategies, as many viruses (e.g., poliovirus, SARS-CoV-2) disrupt SGs to facilitate replication. Purified recombinant G3BP is essential for *in vitro* studies on RNA-protein interactions and high-throughput drug screening.
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