| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FZD7 |
| Uniprot No | O75084 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-574aa |
| 氨基酸序列 | MRDPGAAAPLSSLGLCALVLALLGALSAGAGAQPYHGEKGISVPDHGFCQPISIPLCTDIAYNQTILPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLDQAIPPCRSLCERARQGCEALMNKFGFQWPERLRCENFPVHGAGEICVGQNTSDGSGGPGGGPTAYPTAPYLPDLPFTALPPGASDGRGRPAFPFSCPRQLKVPPYLGYRFLGERDCGAPCEPGRANGLMYFKEEERRFARLWVGVWSVLCCASTLFTVLTYLVDMRRFSYPERPIIFLSGCYFMVAVAHVAGFLLEDRAVCVERFSDDGYRTVAQGTKKEGCTILFMVLYFFGMASSIWWVILSLTWFLAAGMKWGHEAIEANSQYFHLAAWAVPAVKTITILAMGQVDGDLLSGVCYVGLSSVDALRGFVLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEKLEKLMVRIGVFSVLYTVPATIVLACYFYEQAFREHWERTWLLQTCKSYAVPCPPGHFPPMSPDFTVFMIKYLMTMIVGITTGFWIWSGKTLQSWRRFYHRLSHSSKGETAV |
| 分子量 | 88.77 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FZD7蛋白的3篇代表性文献示例(注：内容为示例性概括，实际文献请通过学术数据库查询)：
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1. **文献名称**: *"Recombinant human FZD7 protein activates Wnt/β-catenin signaling and promotes colorectal cancer cell proliferation"*
**作者**: Smith A, et al.
**摘要**: 研究利用HEK293细胞成功表达重组人FZD7胞外结构域蛋白，并证明其通过结合Wnt配体激活β-catenin信号通路，促进结直肠癌细胞增殖，提示FZD7在肿瘤生长中的关键作用。
2. **文献名称**: *"Structural and functional analysis of the FZD7 ectodomain produced in Escherichia coli"*
**作者**: Lee J, Kim H.
**摘要**: 本文通过大肠杆菌系统表达并纯化重组FZD7蛋白的CRD(半胱氨酸富集结构域)，解析其晶体结构，揭示了其与Wnt3a的相互作用界面，为靶向FZD7的药物设计提供结构基础。
3. **文献名称**: *"Recombinant FZD7 suppresses stemness in breast cancer via modulating Wnt signaling"*
**作者**: Zhang Y, et al.
**摘要**: 利用哺乳动物细胞表达系统制备重组FZD7蛋白，发现其通过竞争性抑制内源性FZD7受体功能，阻断Wnt通路，降低乳腺癌干细胞活性，具有潜在治疗价值。
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建议通过PubMed、Web of Science等平台以关键词“recombinant FZD7 protein”或“FZD7 purification”查询最新研究。
**Background of Recombinant Human FZD7 Protein**
Frizzled-7 (FZD7), a member of the Frizzled receptor family, is a critical transmembrane protein that functions as a receptor for Wnt signaling pathways. As a G protein-coupled receptor (GPCR), FZD7 binds Wnt ligands to activate both canonical (β-catenin-dependent) and non-canonical Wnt signaling cascades, regulating essential biological processes such as embryonic development, tissue homeostasis, stem cell maintenance, and cell polarity. Dysregulation of FZD7 has been implicated in various cancers, including colorectal, breast, and hepatocellular carcinomas, where its overexpression often correlates with tumor progression, metastasis, and therapy resistance.
Recombinant human FZD7 protein is engineered using advanced biotechnological methods, such as mammalian or insect cell expression systems, to ensure proper post-translational modifications and functional activity. This purified protein retains the extracellular cysteine-rich domain (CRD), which is vital for Wnt ligand interaction. Researchers utilize recombinant FZD7 to study receptor-ligand binding mechanisms, screen for therapeutic inhibitors, or develop antibody-based therapies targeting Wnt-driven malignancies. Additionally, it serves as a tool in regenerative medicine, aiding investigations into stem cell differentiation and tissue repair.
The development of recombinant FZD7 underscores its therapeutic potential, particularly in disrupting oncogenic Wnt signaling, making it a focal point in drug discovery and personalized cancer treatment strategies.
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