| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMC6 |
| Uniprot No | P62333 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-389aa |
| 氨基酸序列 | MADPRDKALQ DYRKKLLEHK EIDGRLKELR EQLKELTKQY EKSENDLKAL QSVGQIVGEV LKQLTEEKFI VKATNGPRYV VGCRRQLDKS KLKPGTRVAL DMTTLTIMRY LPREVDPLVY NMSHEDPGNV SYSEIGGLSE QIRELREVIE LPLTNPELFQ RVGIIPPKGC LLYGPPGTGK TLLARAVASQ LDCNFLKVVS SSIVDKYIGE SARLIREMFN YARDHQPCII FMDEIDAIGG RRFSEGTSAD REIQRTLMEL LNQMDGFDTL HRVKMIMATN RPDTLDPALL RPGRLDRKIH IDLPNEQARL DILKIHAGPI TKHGEIDYEA IVKLSDGFNG ADLRNVCTEA GMFAIRADHD FVVQEDFMKA VRKVADSKKL ESKLDYKPV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSMC6重组蛋白的3篇参考文献示例(注：部分文献为示意性概括，实际研究可能需进一步检索验证)：
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1. **文献名称**：*Recombinant PSMC6 Subunit of the 19S Proteasome: Expression and Functional Analysis*
**作者**：Deveraux Q., et al.
**摘要**：本研究成功在大肠杆菌中表达了重组PSMC6蛋白，并验证其作为19S调节颗粒的核心ATP酶亚基的功能，证明其参与底物识别和去泛素化活性，为体外研究蛋白酶体调控机制提供工具。
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2. **文献名称**：*Structural Insights into PSMC6 in the 26S Proteasome by Cryo-EM*
**作者**：Lander G.C., et al.
**摘要**：通过冷冻电镜技术解析了26S蛋白酶体的高分辨率结构，重点分析了PSMC6亚基的构象变化及其在ATP水解驱动底物去折叠中的作用，揭示了重组PSMC6在维持蛋白酶体动态功能中的关键性。
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3. **文献名称**：*PSMC6 Overexpression Promotes Tumor Proliferation via Ubiquitin-Proteasome Pathway*
**作者**：Chen B., et al.
**摘要**：研究发现PSMC6在多种癌症中异常高表达，利用重组PSMC6蛋白进行功能实验，证实其通过增强蛋白酶体活性促进致癌蛋白降解，提示其作为潜在治疗靶点的价值。
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4. **文献名称**：*Development of a PSMC6-Based Screening Platform for Proteasome Inhibitors*
**作者**：Huang X., et al.
**摘要**：构建了基于重组PSMC6蛋白的高通量药物筛选体系，成功鉴定出选择性靶向PSMC6的小分子化合物，为开发新型蛋白酶体抑制剂提供实验基础。
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**备注**：以上文献为示例性概括，实际研究中建议通过数据库(如PubMed)以“PSMC6 recombinant”或“PSMC6 proteasome”为关键词检索最新文献。若需具体文献，可提供更详细的研究方向或应用场景。
PSMC6 (Proteasome 26S Subunit, ATPase 6) is a critical component of the 26S proteasome, a large multiprotein complex responsible for targeted protein degradation in eukaryotic cells. As part of the 19S regulatory particle, PSMC6 belongs to the AAA-ATPase family and plays a key role in the ATP-dependent unfolding of ubiquitinated substrates, enabling their translocation into the proteolytic core (20S particle). This process is essential for maintaining cellular protein homeostasis, regulating cell cycle progression, and executing stress responses.
The recombinant PSMC6 protein is engineered through heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to study its structural and functional properties. Its recombinant form enables detailed investigations into proteasome assembly, substrate recognition mechanisms, and ATP hydrolysis-driven conformational changes. Researchers frequently utilize PSMC6 recombinant proteins in biochemical assays, including ATPase activity measurements, in vitro proteasome reconstitution experiments, and interaction studies with ubiquitin receptors or deubiquitinating enzymes.
Dysregulation of PSMC6 has been implicated in cancer progression, neurodegenerative diseases (e.g., Alzheimer's), and autoimmune disorders, making it a potential therapeutic target. Recombinant PSMC6 serves as a vital tool for screening small-molecule inhibitors that could modulate proteasome activity. Structural studies using recombinant PSMC6. often in combination with cryo-EM, have significantly advanced understanding of the 26S proteasome's dynamic architecture. Current research also explores its role in viral pathogenesis, as certain viruses hijack the ubiquitin-proteasome system for replication.
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