| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FZD2 |
| Uniprot No | Q14332 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-565aa |
| 氨基酸序列 | QFHGEKGISIPDHGFCQPISIPLCTDIAYNQTIMPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLEQAIPPCRSICERARQGCEALMNKFGFQWPERLRCEHFPRHGAEQICVGQNHSEDGAPALLTTAPPPGLQPGAGGTPGGPGGGGAPPRYATLEHPFHCPRVLKVPSYLSYKFLGERDCAAPCEPARPDGSMFFSQEETRFARLWILTWSVLCCASTFFTVTTYLVDMQRFRYPERPIIFLSGCYTMVSVAYIAGFVLQERVVCNERFSEDGYRTVVQGTKKEGCTILFMMLYFFSMASSIWWVILSLTWFLAAGMKWGHEAIEANSQYFHLAAWAVPAVKTITILAMGQIDGDLLSGVCFVGLNSLDPLRGFVLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEKLERLMVRIGVFSVLYTVPATIVIACYFYEQAFREHWERSWVSQHCKSLAIPCPAHYTPRMSPDFTVYMIKYLMTLIVGITSGFWIWSGKTLHSWRKFYTRLTNSRHGETTV |
| 分子量 | 62.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条与重组人FZD2蛋白相关的文献示例及摘要概括(注：文献信息为模拟生成，实际引用时请核实准确性)：
1. **"Expression and functional characterization of recombinant human FZD2 in Wnt/β-catenin signaling"**
*作者：Chen L, et al.*
摘要：本研究成功在大肠杆菌中表达并纯化了重组人FZD2胞外结构域，证实其与Wnt3a配体结合后激活经典Wnt信号通路，促进癌细胞增殖。实验通过表面等离子体共振(SPR)分析了FZD2与Wnt蛋白的亲和力。
2. **"Structural insights into FZD2 cysteine-rich domain and its interaction with Norrin ligand"**
*作者：Wang Y, et al.*
摘要：该研究利用X射线晶体学解析了重组hFZD2蛋白的CRD结构域三维结构，揭示其与配体Norrin的结合界面，为遗传性视网膜疾病的靶向治疗提供分子机制依据。
3. **"Recombinant FZD2-Fc fusion protein suppresses tumor growth by antagonizing Wnt signaling in vivo"**
*作者：Kramer I, et al.*
摘要：通过哺乳动物细胞表达系统制备FZD2-Fc重组融合蛋白，证明其可竞争性抑制Wnt1介导的β-catenin活化，并在结肠癌小鼠模型中显著抑制肿瘤生长和转移。
建议通过PubMed或Google Scholar以关键词 **"Recombinant FZD2 protein"** 或 **"FZD2 expression Wnt"** 查询最新实证研究。
Recombinant human FZD2 (Frizzled class receptor 2) protein is a engineered form of the naturally occurring FZD2 receptor, a key component of the Wnt signaling pathway. FZD receptors are integral membrane proteins that interact with Wnt ligands to regulate critical biological processes, including embryonic development, cell proliferation, and tissue homeostasis. FZD2 specifically binds Wnt proteins through its extracellular cysteine-rich domain (CRD), initiating downstream signaling cascades such as the canonical β-catenin-dependent pathway and non-canonical planar cell polarity pathways. Dysregulation of FZD2 has been implicated in cancers, stem cell maintenance, and developmental disorders, making it a research target for therapeutic interventions.
Recombinant FZD2 protein is typically produced in mammalian or insect cell systems to ensure proper post-translational modifications and structural integrity. It retains the functional CRD for ligand binding and is purified for in vitro studies. This protein is widely used to investigate receptor-ligand interactions, Wnt pathway activation mechanisms, and drug screening for FZD2-targeted therapies. Its applications extend to structural biology (e.g., crystallography of Wnt-FZD complexes) and regenerative medicine research, where modulating Wnt signaling could influence tissue repair. Additionally, recombinant FZD2 serves as a critical tool for developing biologics or antibodies to inhibit oncogenic Wnt signaling in cancers.
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