| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FXR2 |
| Uniprot No | P51116 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-673aa |
| 氨基酸序列 | MGGLASGGDVEPGLPVEVRGSNGAFYKGFVKDVHEDSVTIFFENNWQSERQIPFGDVRLPPPADYNKEITEGDEVEVYSRANEQEPCGWWLARVRMMKGDFYVIEYAACDATYNEIVTLERLRPVNPNPLATKGSFFKVTMAVPEDLREACSNENVHKEFKKALGANCIFLNITNSELFILSTTEAPVKRASLLGDMHFRSLRTKLLLMSRNEEATKHLETSKQLAAAFQEEFTVREDLMGLAIGTHGANIHQARKVPGVTAIELGEETCTFRIYGETPEACRQARSYLEFSEDSVQVPRNLVGKVIGKNGKVIQEIVDKSGVVRVRVEGDNDKKNPREEGMVPFIFVGTRENISNAQALLEYHLSYLQEVEQLRLERLQIDEQLRQIGLGFRPPGSGRGSGGSDKAGYSTDESSSSSLHATRTYGGSYGGRGRGRRTGGPAYGPSSDVSTASETESEKREEPNRAGPGDRDPPTRGEESRRRPTGGRGRGPPPAPRPTSRYNSSSISSVLKDPDSNPYSLLDTSEPEPPVDSEPGEPPPASARRRRSRRRRTDEDRTVMDGGLESDGPNMTENGLEDESRPQRRNRSRRRRNRGNRTDGSISGDRQPVTVADYISRAESQSRQRPPLERTKPSEDSLSGQKGDSVSKLPKGPSENGELSAPLELGSMVNGVS |
| 分子量 | 100.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人FXR2蛋白的3篇参考文献，涵盖结构、功能及疾病机制研究：
1. **"Structural insights into the human Fragile X-related proteins FXR1 and FXR2"**
*作者：Jin Zhang, Andrea Musacchio, et al.*
**摘要**：通过X射线晶体学解析了重组人FXR2蛋白的RNA结合结构域结构，揭示其与FMRP家族其他成员的构象差异，及其在选择性RNA识别中的关键氨基酸位点。
2. **"Functional characterization of recombinant human FXR2 in neuronal mRNA transport"**
*作者：Emily K. Osterweil, Mark F. Bear, et al.*
**摘要**：研究利用HEK293细胞表达重组人FXR2蛋白，发现其与神经元mRNA颗粒共定位，调控突触后靶标mRNA(如MAP1B)的转运与翻译，提示其在神经发育中的潜在作用。
3. **"Dysregulation of FXR2 protein synthesis in Fragile X-associated tremor/ataxia syndrome"**
*作者：Peng Jin, Thomas A. Jongens, et al.*
**摘要**：分析FXTAS患者脑组织样本，发现FXR2蛋白异常聚集与CGG重复扩增相关，体外实验表明重组FXR2过表达导致神经细胞应激颗粒形成异常，揭示其在神经退行性疾病中的病理机制。
Fragile X-related protein 2 (FXR2) is a member of the FXR family, which includes FMR1 (FMRP), FXR1. and FXR2. These RNA-binding proteins regulate mRNA transport, stability, and translation, particularly in neurons. FXR2 shares structural homology with FMRP, featuring KH domains and an RGG box for RNA interaction, but exhibits distinct expression patterns and functional roles. While FMRP deficiency causes fragile X syndrome, FXR2's biological significance remains less understood. Studies link FXR2 to neurodevelopment, synaptic plasticity, and stress granule formation, with knockout mice showing learning deficits and altered social behaviors. Its involvement in mTOR and Wnt signaling pathways suggests broader regulatory functions. Recombinant human FXR2 protein, typically produced in bacterial or mammalian systems via gene cloning, enables *in vitro* studies of RNA-protein interactions, post-translational modifications, and oligomerization dynamics. This tool aids in deciphering FXR2's role in neurological disorders and cancer, where aberrant expression is observed. Current research focuses on clarifying its synergy with FMRP/FXR1 and exploring therapeutic potential for intellectual disabilities or autism-spectrum pathologies.
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