| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FYB |
| Uniprot No | O15117 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-829aa |
| 氨基酸序列 | MAKYNTGGNPTEDVSVNSRPFRVTGPNSSSGIQARKNLFNNQGNASPPAGPSNVPKFGSPKPPVAVKPSSEEKPDKEPKPPFLKPTGAGQRFGTPASLTTRDPEAKVGFLKPVGPKPINLPKEDSKPTFPWPPGNKPSLHSVNQDHDLKPLGPKSGPTPPTSENEQKQAFPKLTGVKGKFMSASQDLEPKPLFPKPAFGQKPPLSTENSHEDESPMKNVSSSKGSPAPLGVRSKSGPLKPAREDSENKDHAGEISSLPFPGVVLKPAASRGGPGLSKNGEEKKEDRKIDAAKNTFQSKINQEELASGTPPARFPKAPSKLTVGGPWGQSQEKEKGDKNSATPKQKPLPPLFTLGPPPPKPNRPPNVDLTKFHKTSSGNSTSKGQTSYSTTSLPPPPPSHPASQPPLPASHPSQPPVPSLPPRNIKPPFDLKSPVNEDNQDGVTHSDGAGNLDEEQDSEGETYEDIEASKEREKKREKEEKKRLELEKKEQKEKEKKEQEIKKKFKLTGPIQVIHLAKACCDVKGGKNELSFKQGEQIEIIRITDNPEGKWLGRTARGSYGYIKTTAVEIDYDSLKLKKDSLGAPSRPIEDDQEVYDDVAEQDDISSHSQSGSGGIFPPPPDDDIYDGIEEEDADDGSTLQVQEKSNTWSWGILKMLKGKDDRKKSIREKPKVSDSDNNEGSSFPAPPKQLDMGDEVYDDVDTSDFPVSSAEMSQGTNFGKAKTEEKDLKKLKKQEKEEKDFRKKFKYDGEIRVLYSTKVTTSITSKKWGTRDLQVKPGESLEVIQTTDDTKVLCRNEEGKYGYVLRSYLADNDGEIYDDIADGCIYDND |
| 分子量 | 91.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FYB蛋白的3篇参考文献示例(均为假设性文献，仅供格式参考)：
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1. **文献名称**: "Expression and Functional Analysis of Recombinant Human FYB Protein in T-Cell Signaling"
**作者**: Geng L., et al. (2016)
**摘要**: 研究报道了重组人FYB蛋白在大肠杆菌中的高效表达与纯化，验证其通过调节FYN激酶活性参与T细胞受体(TCR)信号传导，并促进免疫突触形成。
2. **文献名称**: "Structural Insights into FYB Protein Interaction with SH3 Domains"
**作者**: Wang Y., et al. (2018)
**摘要**: 通过X射线晶体学解析了重组人FYB蛋白的结构，揭示了其Src同源3(SH3)结合域的分子机制，为免疫细胞黏附相关疾病提供了潜在靶点。
3. **文献名称**: "CRISPR/Cas9-Mediated FYB Knockout Combined with Recombinant Protein Rescue Reveals Its Role in Neutrophil Migration"
**作者**: Chua R., et al. (2020)
**摘要**: 利用基因编辑技术结合重组人FYB蛋白回补实验，证明FYB通过调控细胞骨架动力学影响中性粒细胞的炎症迁移能力。
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如需真实文献，建议通过PubMed、Google Scholar等平台检索关键词 "recombinant human FYB/ADAP protein" 获取具体信息。
Recombinant human FYB protein, also known as ADAP (Adhesion and Degranulation-promoting Adaptor Protein), is a cytoplasmic adapter protein encoded by the *FYB1* gene. It plays critical roles in immune cell signaling, particularly in T-cell activation and platelet function. FYB contains multiple protein interaction domains, including an SH3 domain-binding motif, proline-rich regions, and tyrosine phosphorylation sites, enabling interactions with signaling molecules like FYN, SKAP1. and SLP-76.
Biologically, FYB regulates integrin activation, cytoskeletal reorganization, and immune synapse formation. Its involvement in TCR (T-cell receptor)-mediated signaling pathways influences T-cell adhesion, cytokine production, and antigen response. Dysregulation of FYB expression or function has been linked to immune disorders, such as autoimmune diseases and immunodeficiencies.
The recombinant form of human FYB protein is typically produced in *E. coli* or mammalian expression systems, enabling in vitro studies of immune signaling mechanisms. Researchers utilize it to investigate T-cell receptor dynamics, immune synapse assembly, and molecular interactions in hematologic or autoimmune conditions. Its applications extend to drug discovery, particularly in targeting pathways associated with aberrant immune responses or thrombosis. The availability of recombinant FYB has significantly advanced structural and functional studies, providing insights into its role as a potential therapeutic target or diagnostic marker.
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