| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCARA3 |
| Uniprot No | Q6AZY7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-606aa |
| 氨基酸序列 | MKVRSAGGDGDALCVTEEDLAGDDEDMPTFPCTQKGRPGPRCSRCQKNLSLHTSVRILYLFLALLLVAVAVLASLVFRKVDSLSEDISLTQSIYDKKLVLMQKNLQGLDPKALNNCSFCHEAGQLGPEIRKLQEELEGIQKLLLAQEVQLDQTLQAQEVLSTTSRQISQEMGSCSFSIHQVNQSLGLFLAQVRGWQATTAGLDLSLKDLTQECYDVKAAVHQINFTVGQTSEWIHGIQRKTDEETLTLQKIVTDWQNYTRLFSGLRTTSTKTGEAVKNIQATLGASSQRISQNSESMHDLVLQVMGLQLQLDNISSFLDDHEENMHDLQYHTHYAQNRTVERFESLEGRMASHEIEIGTIFTNINATDNHVHSMLKYLDDVRLSCTLGFHTHAEELYYLNKSVSIMLGTTDLLRERFSLLSARLDLNVRNLSMIVEEMKAVDTQHGEILRNVTILRGAPGPPGPRGFKGDMGVKGPVGGRGPKGDPGSLGPLGPQGPQGQPGEAGPVGERGPVGPRGFPGLKGSKGSFGTGGPRGQPGPKGDIGPPGPEGPPGSPGPSGPQGKPGIAGKTGSPGQRGAMGPKGEPGIQGPPGLPGPPGPPGSQSFY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SCARA3重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*SCARA3 is a novel oxidative stress-inducible scavenger receptor involved in immune defense*
**作者**：Y. Nakamura et al.
**摘要**：研究发现SCARA3作为清道夫受体家族成员，在氧化应激条件下显著上调，通过结合并清除氧化低密度脂蛋白(oxLDL)减轻细胞氧化损伤，可能参与调控炎症反应和免疫防御。
2. **文献名称**：*Epigenetic silencing of SCARA3 promotes breast cancer progression via ROS/MAPK signaling*
**作者**：H. Oshiumi et al.
**摘要**：该文献揭示SCARA3在乳腺癌中因启动子甲基化而表达沉默，其缺失导致细胞内活性氧(ROS)积累，激活MAPK信号通路促进肿瘤增殖和转移，提示SCARA3可能作为肿瘤抑制因子发挥作用。
3. **文献名称**：*SCARA3 regulates DNA damage response and chemosensitivity in ovarian cancer*
**作者**：Y. Jiang et al.
**摘要**：研究表明SCARA3通过调控ROS水平影响卵巢癌细胞的DNA损伤修复能力，其低表达导致铂类化疗耐药，提示SCARA3可作为预测化疗敏感性的潜在标志物。
注：以上为模拟概括，实际文献需通过PubMed或Google Scholar检索确认。
SCARA3 (Scavenger Receptor Class A Member 3), also known as MARCO (Macrophage Receptor with Collagenous Structure), is a transmembrane protein belonging to the scavenger receptor family. These receptors are characterized by their ability to recognize and internalize modified lipoproteins, pathogens, and cellular debris, playing critical roles in innate immunity, lipid metabolism, and inflammation. SCARA3 is distinguished by its unique structural domains, including a collagen-like triple-helix domain and a cysteine-rich scavenger receptor cysteine-rich (SRCR) domain, which facilitate ligand binding and cellular interactions.
Primarily expressed in macrophages, epithelial cells, and certain tumor-associated cells, SCARA3 is implicated in diverse physiological and pathological processes. It functions as a pattern recognition receptor (PRR), binding to pathogen-associated molecular patterns (PAMPs) and oxidized low-density lipoproteins (oxLDL), thereby contributing to pathogen clearance and modulating inflammatory responses. Studies suggest its involvement in oxidative stress regulation, where it may act as a sensor for damaged molecules, promoting their removal and mitigating tissue damage.
In cancer biology, SCARA3 exhibits dual roles. It can suppress tumor progression by enhancing phagocytosis of apoptotic cells and reducing oxidative stress, but may also promote tumorigenesis in specific contexts by facilitating immune evasion or stromal interactions. Its expression is often dysregulated in cancers, correlating with prognosis and therapeutic resistance.
Recombinant SCARA3 protein is engineered for research and therapeutic applications. Produced via mammalian or insect cell systems to ensure proper post-translational modifications, it retains ligand-binding activity and structural integrity. This recombinant form is utilized to study receptor-ligand interactions, signaling pathways, and its role in disease models. Additionally, it holds potential as a therapeutic agent or target for modulating immune responses, lipid disorders, or cancer microenvironments. Despite progress, SCARA3's precise mechanisms and clinical relevance remain under investigation, highlighting the need for further exploration of its multifunctional biology.
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