纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | BAG3 |
Uniprot No | O95817 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-575aa |
氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MSAATHSPMM QVASGNGDRD PLPPGWEIKI DPQTGWPFFV DHNSRTTTWN DPRVPSEGPK ETPSSANGPS REGSRLPPAR EGHPVYPQLR PGYIPIPVLH EGAENRQVHP FHVYPQPGMQ RFRTEAAAAA PQRSQSPLRG MPETTQPDKQ CGQVAAAAAA QPPASHGPER SQSPAASDCS SSSSSASLPS SGRSSLGSHQ LPRGYISIPV IHEQNVTRPA AQPSFHQAQK THYPAQQGEY QTHQPVYHKI QGDDWEPRPL RAASPFRSSV QGASSREGSP ARSSTPLHSP SPIRVHTVVD RPQQPMTHRE TAPVSQPENK PESKPGPVGP ELPPGHIPIQ VIRKEVDSKP VSQKPPPPSE KVEVKVPPAP VPCPPPSPGP SAVPSSPKSV ATEERAAPST APAEATPPKP GEAEAPPKHP GVLKVEAILE KVQGLEQAVD NFEGKKTDKK YLMIEEYLTK ELLALDSVDP EGRADVRQAR RDGVRKVQTI LEKLEQKAID VPGQVQVYEL QPSNLEADQP LQAIMEMGAV AADKGKKNAG NAEDPHTETQ QPEATAAATS NPSSMTDTPG NPAAP |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BAG3重组蛋白的3篇参考文献及其摘要概括:
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1. **文献名称**: *BAG3-mediated protein homeostasis in cancer*
**作者**: Rosati A. et al.
**摘要**: 研究探讨了BAG3重组蛋白在癌症细胞中的分子伴侣功能,表明其通过结合HSP70调控错误折叠蛋白的清除,并在肿瘤细胞抗凋亡中起关键作用。实验利用重组BAG3验证了其与HSP70的相互作用及对化疗耐药的调控。
2. **文献名称**: *Recombinant BAG3 attenuates mutant huntingtin aggregation through autophagy activation*
**作者**: Xu Z. et al.
**摘要**: 研究通过体外表达BAG3重组蛋白,证明其通过激活自噬途径(如促进LC3-II形成)显著减少亨廷顿病模型中突变蛋白的聚集,揭示了BAG3在神经退行性疾病中的潜在治疗价值。
3. **文献名称**: *Structural insights into BAG3 recognition of HSP70 chaperone*
**作者**: Li J. et al.
**摘要**: 利用重组BAG3蛋白进行结构生物学分析,揭示了其BAG结构域与HSP70 ATP酶域的精确结合位点,阐明了BAG3调控HSP70分子伴侣循环的分子机制。
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以上文献涵盖BAG3重组蛋白在癌症、神经退行性疾病及结构机制中的研究,均涉及重组蛋白的表达与功能验证。如需具体文章,建议通过PubMed或Sci-Hub检索标题获取全文。
BAG3 (Bcl-2-associated athanogene 3) is a multifunctional protein belonging to the BAG family of molecular chaperone regulators. It plays a critical role in cellular stress responses, protein quality control, and apoptosis regulation. Structurally, BAG3 contains a conserved BAG domain that interacts with heat shock protein 70 (Hsp70), modulating its chaperone activity. Additionally, it features a WW domain, PXXP motifs, and a proline-rich region, enabling interactions with other proteins involved in signaling pathways, autophagy, and cytoskeletal organization.
Under stress conditions (e.g., heat shock, oxidative stress), BAG3 expression is upregulated, promoting the selective autophagy of misfolded proteins (aggrephagy) and enhancing cell survival. It also stabilizes pro-survival proteins like Bcl-2 while inhibiting pro-apoptotic factors, positioning it as a key player in balancing cell death and survival. BAG3 is implicated in various diseases, including cancer, neurodegenerative disorders (e.g., Alzheimer’s), and myopathies. In cancer, elevated BAG3 levels correlate with tumor progression, metastasis, and chemotherapy resistance.
Recombinant BAG3 proteins are engineered in vitro using expression systems (e.g., *E. coli*, mammalian cells) for functional studies. These proteins retain critical domains and are used to investigate BAG3’s interactions, mechanisms in stress adaptation, and therapeutic targeting. Researchers employ recombinant BAG3 to screen inhibitors, study pathogenic mutations, or develop strategies to modulate its activity in disease contexts. Its dual role in proteostasis and apoptosis makes it a compelling target for diseases linked to protein aggregation or dysregulated cell survival.
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