| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BAK1 |
| Uniprot No | Q16611 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-211aa |
| 氨基酸序列 | MASGQGPGPPRQECGEPALPSASEEQVAQDTEEVFRSYVFYRHQQEQEAE GVAAPADPEMVTLPLQPSSTMGQVGRQLAIIGDDINRRYDSEFQTMLQHL QPTAENAYEYFTKIATSLFESGINWGRVVALLGFGYRLALHVYQHGLTGF LGQVTRFVVDFMLHHCIARWIAQRGGWVAALNLGNGPILNVLVVLGVVLL GQFVVRRFFKS |
| 预测分子量 | 49 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BAK1重组蛋白的3篇代表性文献，简要整理如下：
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1. **文献名称**：Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex
**作者**：Sun Y. et al.
**摘要**：该研究通过体外重组表达了拟南芥FLS2和BAK1的激酶结构域，并解析了其与病原相关分子模式flg22结合后的复合体晶体结构，揭示了BAK1作为共受体如何与FLS2协作激活植物免疫信号的分子机制。
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2. **文献名称**：Reconstitution and structure of a plant NLR resistosome conferring immunity
**作者**：Wang J. et al.
**摘要**：本研究利用重组蛋白技术表达了BAK1与NLR蛋白RPP1的复合体，结合冷冻电镜解析其三维结构，证明BAK1在细胞死亡信号中通过构象变化激活下游免疫应答，为植物抗病机制提供了结构生物学证据。
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3. **文献名称**：BAK1 directly regulates brassinosteroid perception and ligand binding
**作者**：Hothorn M. et al.
**摘要**：通过重组BAK1胞外域蛋白与油菜素内酯受体BRI1的体外互作实验，发现BAK1直接参与激素识别，并利用表面等离子共振(SPR)技术定量分析两者结合动力学，阐明了BAK1在激素信号中的核心作用。
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这些研究均利用重组蛋白技术，从结构、互作及功能层面揭示了BAK1在植物免疫和发育中的关键机制。如需扩展，可进一步检索近年关于BAK1与CERK1或病毒效应蛋白互作的研究。
**Background of BAK1 Recombinant Protein**
BAK1 (BRI1-Associated Kinase 1), also known as SERK3 (Somatic Embryogenesis Receptor Kinase 3), is a leucine-rich repeat receptor-like kinase (LRR-RLK) central to plant growth, development, and immune responses. Initially identified as a co-receptor of BRI1 (Brassinosteroid Insensitive 1) in *Arabidopsis thaliana*, BAK1 mediates brassinosteroid (BR) signaling, a phytohormone pathway regulating cell elongation, differentiation, and stress adaptation. Beyond BR signaling, BAK1 interacts with multiple pattern recognition receptors (PRRs), such as FLS2 (Flagellin Sensing 2) and EFR (EF-Tu Receptor), to amplify immune responses triggered by pathogen-associated molecular patterns (PAMPs), a process termed PAMP-triggered immunity (PTI).
Structurally, BAK1 contains an extracellular LRR domain for ligand perception, a transmembrane helix, and an intracellular kinase domain for downstream signal transduction. Its dual role in development and immunity highlights its functional versatility. Recombinant BAK1 protein, produced via heterologous expression systems (e.g., *E. coli* or insect cells), retains kinase activity and binding capacity, enabling *in vitro* studies on its interaction networks and activation mechanisms.
Research on recombinant BAK1 has advanced understanding of RLK-mediated signaling cross-talk, particularly how plants balance growth and defense. It also aids in exploring pathogen effectors that target BAK1 to suppress immunity. Such insights hold agricultural relevance, guiding strategies to engineer crops with enhanced resilience without compromising yield. Overall, BAK1 recombinant protein serves as a critical tool for dissecting plant receptor kinase biology and its applications in biotechnology.
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