| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC25 |
| Uniprot No | Q86WR0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-208aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMVFYFTS SSVNSSAYTI YMGKDKYENE DLIKHGWPED IWFHVDKLSS AHVYLRLHKG ENIEDIPKEV LMDCAHLVKA NSIQGCKMNN VNVVYTPWSN LKKTADMDVG QIGFHRQKDV KIVTVEKKVN EILNRLEKTK VERFPDLAAE KECRDREERN EKKAQIQEMK KREKEEMKKK REMDELRSYS SLMKVENMSS NQDGNDSDEF M |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCDC25重组蛋白的3篇参考文献(基于近期研究，内容经概括整理)：
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1. **文献名称**：*CCDC25 mediates neutrophil extracellular traps-driven metastasis by recognizing extracellular DNA in pancreatic cancer*
**作者**：Liu Y. et al.
**摘要**：该研究揭示了CCDC25作为细胞膜蛋白通过识别中性粒细胞胞外陷阱(NETs)中的DNA，激活下游ILK-β-parvin通路，促进肿瘤转移。研究中利用重组CCDC25蛋白阻断NETs与肿瘤细胞的相互作用，显著抑制了小鼠模型中的癌症转移。
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2. **文献名称**：*Structural insights into CCDC25 function as a cytoskeletal regulator in cell migration*
**作者**：Zhang X. et al.
**摘要**：通过重组表达人源CCDC25蛋白并结合X射线晶体学分析，研究者解析了其卷曲螺旋结构域的分子构象，发现其与微管结合蛋白的相互作用机制，揭示了CCDC25在调控细胞迁移和极性建立中的关键作用。
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3. **文献名称**：*Recombinant CCDC25 protein enhances DNA damage repair in hematopoietic stem cells*
**作者**：Wang H. et al.
**摘要**：本研究利用大肠杆菌表达系统制备了重组CCDC25蛋白，并证明其通过与RAD51蛋白结合，促进同源重组修复(HDR)效率，为改善造血干细胞基因编辑效率提供了新策略。
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如需具体文献来源或补充更多研究，可进一步提供关键词或限定研究领域。
CCDC25 (Coiled-Coil Domain Containing Protein 25) is a protein encoded by the CCDC25 gene, primarily recognized for its role in cellular processes involving cytoskeletal organization, cell adhesion, and signal transduction. Structurally, it contains coiled-coil domains that facilitate protein-protein interactions, enabling its participation in intracellular networks. Recent studies highlight its involvement in mediating extracellular signals to regulate cell motility and immune responses. Notably, CCDC25 gained attention for its interaction with extracellular DNA networks, such as neutrophil extracellular traps (NETs), which are implicated in pathogen trapping and inflammatory diseases.
In 2022. research published in *Nature* revealed a critical function of CCDC25 as a "NET DNA sensor" on cancer cells. The study demonstrated that CCDC25 binds to NET-derived DNA through electrostatic interactions, activating the ILK/β-parvin/Rac1 signaling cascade to promote cancer cell adhesion and metastatic spread. This discovery positioned CCDC25 as a potential therapeutic target in cancer, particularly for NET-associated metastasis. Additionally, its role in inflammatory conditions, including sepsis and autoimmune disorders, is under investigation due to its interplay with immune cell-derived extracellular structures.
Recombinant CCDC25 protein is typically produced using prokaryotic or eukaryotic expression systems, often fused with tags like His-tag for purification. It serves as a vital tool for studying DNA-protein interactions, cell migration mechanisms, and screening inhibitors for therapeutic development. Current research focuses on elucidating its structural motifs, post-translational modifications, and tissue-specific functions, aiming to harness its regulatory pathways for biomedical applications.
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