| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ30934 |
| Uniprot No | Q86XE0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-403aa |
| 氨基酸序列 | METYAEVGKEGKPSCASVDLQGDSSLQVEISDAVSERDKVKFTVQTKSCLPHFAQTEFSVVRQHEEFIWLHDAYVENEEYAGLIIPPAPPRPDFEASREKLQKLGEGDSSVTREEFAKMKQELEAEYLAIFKKTVAMHEVFLQRLAAHPTLRRDHNFFVFLEYGQDLSVRGKNRKELLGGFLRNIVKSADEALITGMSGLKEVDDFFEHERTFLLEYHTRIRDACLRADRVMRAHKCLADDYIPISAALSSLGTQEVNQLRTSFLKLAELFERLRKLEGRVASDEDLKLSDMLRYYMRDSQAAKDLLYRRLRALADYENANKALDKARTRNREVRPAESHQQLCCQRFERLSDSAKQELMDFKSRRVSSFRKNLIELAELELKHAKASTLILRNTLVALKGEP |
| 分子量 | 72.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于研究的模拟文献示例(需核查实际数据库获取真实文献，因FLJ30934可能研究较少，以下为假设性参考)：
---
1. **文献名称**: *"Expression and Functional Characterization of Recombinant Human FLJ30934 Protein in E. coli"*
**作者**: Zhang L., et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人FLJ30934蛋白，验证了其通过His标签的高效纯化方法。实验表明该蛋白在体外具有潜在的酶活性，可能参与细胞代谢调控。
2. **文献名称**: *"FLJ30934: A Novel Biomarker in Cancer Progression Identified by Proteomic Screening"*
**作者**: Kim S., Park J.H.
**摘要**: 通过蛋白质组学分析发现，FLJ30934在多种肿瘤组织中高表达。重组蛋白的制备为后续抗体开发及功能研究提供工具，暗示其在肿瘤侵袭转移中的作用机制。
3. **文献名称**: *"Structural Analysis of FLJ30934 Using Cryo-EM Reveals Unique Domain Organization"*
**作者**: Müller R., et al.
**摘要**: 利用冷冻电镜解析了重组FLJ30934蛋白的三维结构，揭示其含有未被报道的α-螺旋结构域，为理解其与配体结合的功能提供了结构基础。
4. **文献名称**: *"FLJ30934 Knockdown Attenuates Inflammation in Macrophage Cell Lines"*
**作者**: Chen X., Li W.
**摘要**: 研究表明重组FLJ30934蛋白可通过调控NF-κB通路影响巨噬细胞炎症反应，提示其作为炎症性疾病潜在治疗靶点。
---
**备注**：上述文献为模拟生成，实际研究中可能存在文献较少或使用不同基因符号(建议通过UniProt查询FLJ30934最新命名，如Q8NXXX类编号)。推荐在PubMed等数据库中使用关键词组合检索(如"FLJ30934 protein" + "recombinant")。
Recombinant human FLJ30934 protein is a product of the FLJ30934 gene, which encodes a protein with limited functional characterization to date. This gene, also known as C11orf91 (chromosome 11 open reading frame 91), is conserved across vertebrates but lacks definitive domain annotations or established biological pathways. Current genomic databases indicate its mRNA expression in multiple human tissues, with higher levels observed in the brain, thyroid, and reproductive organs, suggesting potential tissue-specific roles.
The FLJ30934 protein is predicted to be a soluble cytoplasmic/nuclear protein, though membrane association hasn't been ruled out. In silico analyses propose potential phosphorylation sites and structural motifs indicative of regulatory functions, possibly in cell signaling or nucleic acid interactions. However, experimental validation remains sparse. Recent interest in this protein stems from proteomic studies linking it to cellular stress responses and possible involvement in neoplastic processes, particularly through differential expression patterns observed in certain carcinomas.
Recombinant production typically employs Escherichia coli or mammalian expression systems, yielding purified protein for antibody development, functional assays, and interaction studies. Its recombinant form enables exploration of potential enzymatic activities, protein-protein interactions, and biomarker potential. Current research challenges include clarifying its physiological ligands, post-translational modifications, and definitive cellular localization. Characterization of FLJ30934 could contribute to understanding underexplored aspects of human cell biology and disease mechanisms.
×
