| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ25084 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-152aa |
| 氨基酸序列 | MGKGYYLKGKIGKVPVRFLVDSGAQVSVVHPNLWEEVTDGDLDTLQPFENVVKVANGAEMKILGVWDTAVSLGKLKLKAQFLVANASAEEAIIGTDVLQDHNAILDFEHRTCTLKGKKFRLLPVGGSLEDEFDLELIEEDPSSEEGRQELSH |
| 分子量 | 43.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FLJ25084蛋白的参考文献示例(**注：部分内容为模拟虚构，仅作格式参考**)：
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1. **文献名称**: *FLJ25084 promotes hepatocellular carcinoma progression by regulating RhoA signaling*
**作者**: Zhang L, et al.
**摘要**: 本研究通过重组人FLJ25084蛋白体外表达，发现其通过激活RhoA信号通路促进肝癌细胞迁移和侵袭。蛋白互作实验表明，FLJ25084与RhoGAP家族成员存在相互作用，提示其在细胞骨架重塑中的潜在功能。
2. **文献名称**: *Recombinant expression and enzymatic characterization of FLJ25084 as a novel GTPase-activating protein*
**作者**: Wang Y, et al.
**摘要**: 该研究成功在大肠杆菌中重组表达并纯化人FLJ25084蛋白，证实其具有GTP酶激活活性(GAP)，可加速Rho家族GTP酶的水解过程，为后续结构功能研究提供基础。
3. **文献名称**: *FLJ25084 regulates neural progenitor proliferation during cortical development*
**作者**: Takahashi N, et al.
**摘要**: 利用重组FLJ25084蛋白处理小鼠神经干细胞，发现其显著增强细胞增殖能力，并通过抑制Notch信号通路影响皮层神经元分化，表明其在神经发育中的关键作用。
4. **文献名称**: *Proteomic analysis of FLJ25084-interacting proteins in colorectal cancer*
**作者**: Liu H, et al.
**摘要**: 通过重组FLJ25084蛋白的亲和层析技术筛选结直肠癌细胞中相互作用蛋白，发现其与β-catenin和APC复合物相关，提示其可能参与Wnt通路调控。
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**注意**：上述文献为假设性示例，实际研究中请通过权威数据库(如PubMed、Web of Science)核实具体内容。若研究较少，建议拓展检索FLJ25084的基因别名(如ARHGAP11A)或相关功能关键词。
Recombinant human FLJ25084 protein, also known as ADP-ribosylation factor-like 6 interacting protein 1 (ARL6IP1), is a poorly characterized protein encoded by the ARL6IP1 gene. Initially identified through cDNA cloning as a hypothetical protein (FLJ25084), it belongs to the ARL6IP family and is conserved across eukaryotes. The protein localizes to the endoplasmic reticulum and nuclear membrane, featuring multiple transmembrane domains and potential post-translational modification sites. Its exact biological function remains unclear, though studies suggest roles in vesicular trafficking, endoplasmic reticulum stress response, and apoptosis regulation. Interactions with ARL6 (ADP-ribosylation factor-like 6) and other trafficking-related proteins imply participation in membrane transport processes. Recombinant versions are typically expressed in E. coli or mammalian systems for biochemical studies, often tagged for purification. Emerging research links ARL6IP1 to neurological conditions, including hereditary spastic paraplegia, and cancer progression through unclear mechanisms. Current applications involve using recombinant FLJ25084/ARL6IP1 in functional assays, antibody development, and exploring its potential as a diagnostic marker or therapeutic target, though further characterization is needed to fully elucidate its physiological and pathological significance.
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