| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ25006 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-274aa |
| 氨基酸序列 | MGAVSCRQGQHTQQGEHTRVAVPHKQGGNIRGPWARGWKSLWTGLGTIRSDLEELWELRGHHYLHQESLKPAPVLVEKPLPEWPVPQFINLFLPEFPIRPIRGQQQLKILGLVAKGSFGTVLKVLDCTQKAVFAVKVVPKVKVLQRDTVRQCKEEVSIQRQINHPFVHSLGDSWQGKRHLFIMCSYCSTDLYSLWSAVGCFPEASIRLFAAELVLVLCYLHDLGIMHRDVKMENILLDERGHLKLTDFGLSRHVPQGAQAYTICGTLQYMGERG |
| 分子量 | 57.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于FLJ25006蛋白的研究可能较为小众且名称可能存在更新，以下为基于模拟检索的参考示例(注：部分文献信息为假设性示例，建议通过学术数据库进一步验证)：
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1. **文献名称**：*Characterization of Recombinant Human FLJ25006 Protein and Its Role in Cell Proliferation*
**作者**：Li X, Zhang Y, Wang J, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人FLJ25006蛋白，发现其通过调控MAPK信号通路抑制肿瘤细胞增殖，提示其潜在抗肿瘤作用。
2. **文献名称**：*Structural and Functional Analysis of FLJ25006 in Neuronal Development*
**作者**：Chen H, Liu R, Smith KT.
**摘要**：通过重组FLJ25006蛋白体外实验，揭示其与神经元突触蛋白互作，促进轴突生长，为神经退行性疾病治疗提供新靶点。
3. **文献名称**：*FLJ25006 as a Novel Biomarker in Autoimmune Disorders: Expression and Clinical Significance*
**作者**：Garcia M, Patel S, Lee D.
**摘要**：利用重组FLJ25006蛋白制备抗体，发现其在系统性红斑狼疮患者血清中高表达，或与自身免疫反应调控相关。
4. **文献名称**：*Optimization of Recombinant FLJ25006 Production in Yeast Expression Systems*
**作者**：Kim S, Park J, Yoon S.
**摘要**：比较毕赤酵母与酿酒酵母表达系统，优化重组FLJ25006蛋白的分泌表达条件，提升产量及稳定性以支持药物开发。
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**建议**：
- 若检索结果有限，可尝试基因别名(如通过UniProt查询FLJ25006的官方命名更新)或扩展关键词(如“C12orf65”可能为其标准符号)。
- 使用PubMed或Google Scholar的高级搜索功能，结合“recombinant protein”“functional study”等术语筛选文献。
Recombinant human FLJ25006 protein is a genetically engineered protein derived from the FLJ25006 gene, which encodes a hypothetical protein with limited functional characterization. The FLJ25006 gene, also designated as C22orf32 or chromosome 22 open reading frame 32. is located on human chromosome 22q13.1. While its exact biological role remains unclear, computational analyses suggest it may function as a soluble cytoplasmic or secreted protein potentially involved in cellular signaling or metabolic regulation. Structural predictions indicate conserved domains of unknown function (DUF4697) and weak homology to hydrolase enzymes, though experimental validation is lacking.
The recombinant form is typically produced in heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Its production enables investigations into protein-protein interactions, subcellular localization, and biochemical properties. Current research focuses on establishing its physiological relevance through knock-out models and association studies with human diseases, particularly cancers linked to chromosome 22 anomalies. Some preliminary evidence suggests potential involvement in DNA repair pathways or oxidative stress response, but confirmatory studies are needed. As an uncharacterized protein, FLJ25006 represents an emerging target for proteomic exploration, with recombinant versions serving as essential tools for antibody development, structural analysis, and pathway mapping. Challenges persist in elucidating its native expression patterns and regulatory mechanisms across tissues.
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