| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BCOR |
| Uniprot No | Q6W2J9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BCOR重组蛋白的3篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**：*Recurrent mutations in the BCOR gene in clear cell sarcoma of the kidney*
**作者**：Roy A, et al.
**摘要概括**：
该研究在肾透明细胞肉瘤(CCSK)中发现BCOR基因的重复突变，并通过重组蛋白表达实验证实突变导致BCOR蛋白功能异常，破坏其与Polycomb抑制复合物(PRC1.1)的相互作用，从而影响染色质修饰和致癌通路。
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2. **文献名称**：*BCOR regulates mesenchymal stem cell function by epigenetic mechanisms*
**作者**：Wang Z, et al.
**摘要概括**：
研究利用重组BCOR蛋白体外模型，揭示其通过表观遗传机制调控间充质干细胞的增殖和分化。BCOR通过结合组蛋白去乙酰化酶复合物(HDACs)，抑制成骨相关基因表达，维持干细胞多能性。
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3. **文献名称**：*Structural and functional analysis of BCOR mutations in myeloid malignancies*
**作者**：Grossmann V, et al.
**摘要概括**：
该研究对髓系白血病中的BCOR突变进行功能分析，通过重组BCOR蛋白体外实验发现，截短型突变体丧失转录抑制活性，导致造血干/祖细胞异常增殖，证实BCOR在血液系统肿瘤中的抑癌作用。
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**备注**：若需更多文献或具体DOI信息，可进一步在PubMed或Web of Science中检索关键词“BCOR recombinant protein”或“BCOR functional analysis”。
**Background on BCOR Recombinant Protein**
The BCL-6 corepressor (BCOR) is a transcriptional regulatory protein encoded by the *BCOR* gene, located on the X chromosome. It functions as a critical component of epigenetic modulation, primarily through its role in polycomb repressive complex 1 (PRC1.1), a chromatin-modifying complex. BCOR interacts with BCL-6. a transcriptional repressor involved in lymphocyte differentiation, and other partners to regulate gene expression by modifying histone marks (e.g., H2A ubiquitination) and stabilizing chromatin structure. This activity is essential for embryonic development, stem cell maintenance, and tissue-specific differentiation.
BCOR recombinant protein is engineered to study its molecular mechanisms and interactions. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant form retains functional domains, including the BCL-6 binding motif, ankyrin repeats, and PUFD domain, enabling in vitro studies on its repressive activity, protein-protein interactions, and structural properties. Researchers utilize it to dissect pathways linked to BCOR-associated diseases, such as somatic mutations in cancers (e.g., clear cell sarcoma, leukemia) and germline mutations in developmental disorders like oculo-facio-cardio-dental (OFCD) syndrome.
Interest in BCOR has surged due to its dual role as a tumor suppressor and oncogenic driver, depending on cellular context. Recombinant BCOR tools aid in exploring its interplay with signaling pathways (e.g., Wnt/β-catenin) and developing targeted therapies. Additionally, its involvement in maintaining stem cell pluripotency highlights potential applications in regenerative medicine. Overall, BCOR recombinant protein serves as a vital reagent for unraveling its biological significance and translational potential.
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