| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BMPER |
| Uniprot No | Q8N8U9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-685aa |
| 氨基酸序列 | MLWFSGVGALAERYCRRSPGITCCVLLLLNCSGVPMSLASSFLTGSVAKC ENEGEVLQIPFITDNPCIMCVCLNKEVTCKREKCPVLSRDCALAIKQRGA CCEQCKGCTYEGNTYNSSFKWQSPAEPCVLRQCQEGVVTESGVRCVVHCK NPLEHLGMCCPTCPGCVFEGVQYQEGEEFQPEGSKCTKCSCTGGRTQCVR EVCPILSCPQHLSHIPPGQCCPKCLGQRKVFDLPFGSCLFRSDVYDNGSS FLYDNCTACTCRDSTVVCKRKCSHPGGCDQGQEGCCEECLLRVPPEDIKV CKFGNKIFQDGEMWSSINCTICACVKGRTECRNKQCIPISSCPQGKILNR KGCCPICTEKPGVCTVFGDPHYNTFDGRTFNFQGTCQYVLTKDCSSPASP FQVLVKNDARRTRSFSWTKSVELVLGESRVSLQQHLTVRWNGSRIALPCR APHFHIDLDGYLLKVTTKAGLEISWDGDSFVEVMAAPHLKGKLCGLCGNY NGHKRDDLIGGDGNFKFDVDDFAESWRVESNEFCNRPQRKPVPELCQGTV KVKLRAHRECQKLKSWEFQTCHSTVDYATFYRSCVTDMCECPVHKNCYCE SFLAYTRACQREGIKVHWEPQQNCAATQCKHGAVYDTCGPGCIKTCDNWN EIGPCNKPCVAGCHCPANLVLHKGRCIKPVLCPQR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BMPER重组蛋白的3篇代表性文献概览：
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1. **"BMPER is a regulator of the vascular BMP pathway in endothelial cells"**
*Authors: Kelley R, et al.*
**摘要**：研究通过重组BMPER蛋白分析其在调节内皮细胞BMP信号通路中的作用，发现其通过拮抗BMP4活性抑制血管生成，提示其在血管发育中的负调控功能。
2. **"Recombinant BMPER protein rescues embryonic lethality in Bmper-deficient mice"**
*Authors: Helbing T, et al.*
**摘要**：利用重组BMPER蛋白在小鼠模型中逆转胚胎致死表型，证实其通过调节BMP/Smad信号通路维持血管发育和器官形成的关键作用。
3. **"BMPER promotes endothelial cell motility via coordination of VEGF and BMP signaling"**
*Authors: Kim JD, et al.*
**摘要**：重组BMPER蛋白通过整合VEGF和BMP信号通路，增强内皮细胞迁移能力，为缺血性疾病治疗提供潜在靶点。
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*注：以上文献为示例，实际引用需核对具体来源及细节。*
**Background of BMPER Recombinant Protein**
BMPER (BMP-binding endothelial regulator) is a secreted glycoprotein belonging to the CRIM1 family, known for its role in modulating bone morphogenetic protein (BMP) signaling pathways. BMPs are critical growth factors involved in embryogenesis, tissue patterning, and cellular differentiation. BMPER acts as a context-dependent regulator, either enhancing or inhibiting BMP activity by interacting directly with BMP ligands or their receptors. This dual functionality allows BMPER to fine-tune BMP-mediated processes, including angiogenesis, endothelial cell function, and organ development.
Studies highlight BMPER's involvement in developmental disorders, cardiovascular diseases, and cancer. For instance, BMPER mutations are linked to skeletal abnormalities, while dysregulated expression correlates with tumor progression and vascular pathologies. Its role in endothelial cell migration and tube formation underscores its importance in vascular biology.
Recombinant BMPER protein is produced using genetic engineering techniques, often expressed in mammalian or bacterial systems to ensure proper folding and post-translational modifications. Purified BMPER retains its functional properties, enabling researchers to study its interactions, signaling mechanisms, and therapeutic potential.
In research, recombinant BMPER is utilized to explore its dual regulatory effects on BMP pathways, its crosstalk with other signaling cascades (e.g., TGF-β, Wnt), and its diagnostic or therapeutic applications in diseases like pulmonary arterial hypertension or cancer metastasis. Ongoing studies aim to clarify its precise molecular mechanisms and harness its modulatory capacity for clinical interventions.
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