| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BMX |
| Uniprot No | P51813 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-675aa |
| 氨基酸序列 | MDTKSILEELLLKRSQQKKKMSPNNYKERLFVLTKTNLSYYEYDKMKRGS RKGSIEIKKIRCVEKVNLEEQTPVERQYPFQIVYKDGLLYVYASNEESRS QWLKALQKEIRGNPHLLVKYHSGFFVDGKFLCCQQSCKAAPGCTLWEAYA NLHTAVNEEKHRVPTFPDRVLKIPRAVPVLKMDAPSSSTTLAQYDNESKK NYGSQPPSSSTSLAQYDSNSKKIYGSQPNFNMQYIPREDFPDWWQVRKLK SSSSSEDVASSNQKERNVNHTTSKISWEFPESSSSEEEENLDDYDWFAGN ISRSQSEQLLRQKGKEGAFMVRNSSQVGMYTVSLFSKAVNDKKGTVKHYH VHTNAENKLYLAENYCFDSIPKLIHYHQHNSAGMITRLRHPVSTKANKVP DSVSLGNGIWELKREEITLLKELGSGQFGVVQLGKWKGQYDVAVKMIKEG SMSEDEFFQEAQTMMKLSHPKLVKFYGVCSKEYPIYIVTEYISNGCLLNY LRSHGKGLEPSQLLEMCYDVCEGMAFLESHQFIHRDLAARNCLVDRDLCV KVSDFGMTRYVLDDQYVSSVGTKFPVKWSAPEVFHYFKYSSKSDVWAFGI LMWEVFSLGKQPYDLYDNSQVVLKVSQGHRLYRPHLASDTIYQIMYSCWH ELPEKRPTFQQLLSSIEPLREKDKH |
| 预测分子量 | 110 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与BMX重组蛋白相关的参考文献示例(注：内容为示例性概括，非真实文献)：
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1. **文献名称**: *BMX/ETK kinase regulates osteoclast differentiation by promoting cell migration and survival*
**作者**: Chen H, et al.
**摘要**: 本研究通过重组BMX蛋白体外实验，揭示了BMX在破骨细胞分化中的双重作用：其激酶活性通过激活PI3K/AKT信号通路增强细胞迁移能力，同时抑制凋亡蛋白Caspase-3.从而促进破骨细胞存活。
2. **文献名称**: *Structural basis of BMX kinase activation by growth factor receptors*
**作者**: Qiu Y, Jiang J
**摘要**: 通过解析重组BMX激酶结构域与表皮生长因子受体(EGFR)胞内段的复合物晶体结构，发现BMX的SH2结构域特异性结合EGFR磷酸化酪氨酸位点，揭示了其被受体激酶招募并激活的分子机制。
3. **文献名称**: *Targeting BMX in tumor-associated macrophages enhances anti-PD-1 immunotherapy*
**作者**: Wang L, et al.
**摘要**: 研究利用重组BMX蛋白筛选小分子抑制剂，发现抑制BMX可重塑肿瘤微环境，降低M2型巨噬细胞极化，联合PD-1抗体显著增强小鼠模型中的抗肿瘤免疫应答。
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以上示例结合了BMX重组蛋白在结构解析、功能研究及治疗应用中的典型方向，供参考使用。实际文献需通过学术数据库检索确认。
BMX (Bone Marrow Tyrosine Kinase on the X Chromosome), also known as ETK, is a non-receptor tyrosine kinase belonging to the TEC kinase family. It plays a critical role in intracellular signaling pathways regulating cell proliferation, survival, migration, and angiogenesis. BMX is primarily expressed in bone marrow, endothelial cells, and epithelial tissues, and is activated by stimuli such as cytokines, growth factors, and stress signals. Its structure includes a pleckstrin homology (PH) domain for membrane targeting, Src homology (SH2 and SH3) domains for protein interactions, and a catalytic kinase domain. BMX interacts with signaling cascades like PI3K/Akt, STAT, and NF-κB, contributing to immune responses, vascular development, and cancer progression.
Recombinant BMX proteins are engineered using expression systems (e.g., bacterial, insect, or mammalian cells) to produce purified, functional kinase domains or full-length proteins for research. These tools enable studies on BMX’s enzymatic activity, substrate specificity, and regulatory mechanisms. In disease contexts, BMX is implicated in solid tumors (e.g., prostate, breast cancers) and hematologic malignancies by promoting oncogenic signaling and therapy resistance. It also influences inflammatory diseases and vascular disorders through endothelial cell regulation. Recombinant BMX is vital for high-throughput drug screening to identify inhibitors, with therapeutic potential in oncology and immune dysregulation. Current research focuses on elucidating its role in tumor microenvironments and optimizing BMX-targeted therapies to improve precision medicine approaches.
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