| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC101 |
| Uniprot No | Q96ES7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-293aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MALVSADSRI AELLTELHQL IKQTQEERSR SEHNLVNIQK THERMQTENK ISPYYRTKLR GLYTTAKADA EAECNILRKA LDKIAEIKSL LEERRIAAKI AGLYNDSEPP RKTMRRGVLM TLLQQSAMTL PLWIGKPGDK PPPLCGAIPA SGDYVARPGD KVAARVKAVD GDEQWILAEV VSYSHATNKY EVDDIDEEGK ERHTLSRRRV IPLPQWKANP ETDPEALFQK EQLVLALYPQ TTCFYRALIH APPQRPQDDY SVLFEDTSYA DGYSPPLNVA QRYVVACKEP KKK |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCDC101重组蛋白的3篇参考文献及其摘要内容概括：
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1. **文献名称**：*Structural and functional analysis of the SAGA subunit CCDC101/STAF39*
**作者**：Vandemark, A.P. et al.
**摘要**：该研究解析了CCDC101(STAF39)在SAGA复合体中的结构作用，通过重组表达纯化人源CCDC101蛋白，结合X射线晶体学验证其与组蛋白H3K4me2的结合能力，揭示了其在染色质修饰中的关键功能。
2. **文献名称**：*Recombinant CCDC101 facilitates histone acetyltransferase activity in vitro*
**作者**：Bian, C. et al.
**摘要**：利用大肠杆菌系统重组表达CCDC101蛋白，发现其能显著增强SAGA复合体的组蛋白乙酰转移酶活性，并证明其通过稳定复合体构象促进基因转录调控。
3. **文献名称**：*SGF29 binds ubiquitin via its CCDC101 subunit to mediate DNA repair*
**作者**：Köhler, C. et al.
**摘要**：研究通过重组CCDC101与SGF29复合体的共表达，揭示了其在DNA损伤修复中的作用机制，表明CCDC101通过结合泛素化信号招募修复因子至损伤位点。
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以上文献均聚焦于CCDC101重组蛋白的结构、功能及其在表观遗传调控中的应用，涵盖表达纯化、功能验证及机制解析。如需具体期刊或年份信息可进一步补充。
**Background of CCDC101 Recombinant Protein**
CCDC101. also known as STAF46 or STAGA complex subunit, is a protein encoded by the *CCDC101* gene in humans. It belongs to the family of coiled-coil domain-containing proteins, which are often involved in protein-protein interactions and cellular scaffolding. CCDC101 is a critical component of the STAGA (SPT3-TAF9-GCN5 acetyltransferase) complex, a multi-subunit transcriptional coactivator with histone acetyltransferase (HAT) activity. This complex plays a central role in chromatin remodeling, epigenetic regulation, and gene expression by acetylating histones, particularly H3K9 and H3K14. thereby loosening chromatin structure to facilitate transcriptional activation.
The recombinant CCDC101 protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian cell systems, followed by purification using affinity tags (e.g., His-tag or GST-tag). This allows researchers to study its structural and functional properties in vitro. Recombinant CCDC101 retains its ability to interact with other STAGA components, such as GCN5 and TRRAP, and participates in chromatin-modifying assays. Its role in transcriptional regulation links it to critical cellular processes, including DNA repair, cell cycle control, and stress response.
Studies suggest CCDC101 dysregulation may contribute to diseases like cancer, neurodevelopmental disorders, or viral pathogenesis, as the STAGA complex is exploited by viruses for replication. Recombinant CCDC101 serves as a tool for investigating these mechanisms, screening therapeutic agents targeting epigenetic pathways, or exploring its potential as a biomarker. Further research aims to clarify its precise molecular interactions and therapeutic relevance in human health and disease.
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