| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RHOA |
| Uniprot No | P61586 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-190aa |
| 氨基酸序列 | MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDG KQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWT PEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANR IGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGC |
| 预测分子量 | 23kDa. |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RHOA重组蛋白研究的3篇代表性文献，简要整理供参考：
1. **文献名称**：Expression, purification, and biochemical characterization of recombinant RHOA protein
**作者**：Smith J, et al.
**摘要**：该研究报道了在大肠杆菌中高效表达可溶性RHOA重组蛋白的优化方法，通过His标签亲和层析纯化，并验证其GTP结合活性及与下游效应分子(如ROCK)的相互作用。
2. **文献名称**：Structural insights into RHOA activation and signaling mechanisms
**作者**：Chen L, et al.
**摘要**：利用重组RHOA蛋白进行X射线晶体学研究，解析了其与GEF(鸟嘌呤核苷酸交换因子)复合物的结构，揭示了RHOA从GDP结合态向GTP结合态转变的分子机制。
3. **文献名称**：Functional characterization of RHOA mutants in cancer cell migration
**作者**：Tanaka K, et al.
**摘要**：通过体外重组RHOA蛋白(野生型及常见致癌突变体)的功能分析，证明特定突变体(如G17V)导致组成型激活，显著增强肿瘤细胞侵袭能力，为靶向治疗提供依据。
注：以上为模拟文献案例，实际研究中建议通过PubMed/Google Scholar检索关键词(如"recombinant RHOA protein purification"、"RHOA signaling structural analysis")获取最新文献。如需具体文献推荐，请补充研究背景需求(如蛋白表达系统或功能研究方向)。
**Background of RHOA Recombinant Protein**
RHOA (Ras Homolog Family Member A) is a small GTPase belonging to the Rho subfamily of the Ras superfamily. It functions as a molecular switch, cycling between an active GTP-bound state and an inactive GDP-bound state, regulated by guanine nucleotide exchange factors (GEFs), GTPase-activating proteins (GAPs), and guanine nucleotide dissociation inhibitors (GDIs). RHOA plays a critical role in regulating cytoskeletal dynamics, cell adhesion, migration, proliferation, and apoptosis. It influences actin stress fiber formation, focal adhesion assembly, and contractility through downstream effectors like ROCK (Rho-associated kinase), mDia (mammalian Diaphanous), and other signaling cascades.
Recombinant RHOA protein is engineered for in vitro studies to elucidate its structure-function relationships, interaction networks, and role in cellular pathways. Produced using expression systems such as *E. coli* or mammalian cells, the recombinant protein retains functional domains necessary for GTP/GDP binding and effector interactions. It is commonly utilized in biochemical assays (e.g., GTPase activity assays, pull-down experiments), structural studies (e.g., crystallography), and cell-based experiments to investigate RHOA-mediated signaling.
Dysregulation of RHOA is implicated in various diseases, including cancer, cardiovascular disorders, and neurological conditions. For example, RHOA hyperactivity is linked to tumor invasion and metastasis, while its loss of function correlates with impaired vascular development. Recombinant RHOA variants, including constitutively active (e.g., G14V) or dominant-negative (e.g., T19N) mutants, are pivotal tools for dissecting its pathological mechanisms and screening therapeutic compounds.
Overall, RHOA recombinant protein serves as a cornerstone for advancing research into Rho GTPase biology and its translational applications in human health and disease.
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