| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHEK1 |
| Uniprot No | O14757 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-476aa |
| 氨基酸序列 | MAVPFVEDWD LVQTLGEGAY GEVQLAVNRV TEEAVAVKIV DMKRAVDCPE NIKKEICINK MLNHENVVKF YGHRREGNIQ YLFLEYCSGG ELFDRIEPDI GMPEPDAQRF FHQLMAGVVY LHGIGITHRD IKPENLLLDE RDNLKISDFG LATVFRYNNR ERLLNKMCGT LPYVAPELLK RREFHAEPVD VWSCGIVLTA MLAGELPWDQ PSDSCQEYSD WKEKKTYLNP WKKIDSAPLA LLHKILVENP SARITIPDIK KDRWYNKPLK KGAKRPRVTS GGVSESPSGF SKHIQSNLDF SPVNSASSEE NVKYSSSQPE PRTGLSLWDT SPSYIDKLVQ GISFSQPTCP DHMLLNSQLL GTPGSSQNPW QRLVKRMTRF FTKLDADKSY QCLKETCEKL GYQWKKSCMN QVTISTTDRR NNKLIFKVNL LEMDDKILVD FRLSKGDGLE FKRHFLKIKG KLIDIVSSQK IWLPAT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CHEK1重组蛋白的3-4篇文献示例(内容基于公开研究整理，具体信息需核实)：
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1. **文献名称**：*"Expression and Purification of Active Recombinant Human CHK1 Kinase"*
**作者**：Katsuya, Y. et al.
**摘要**：该研究报道了通过大肠杆菌表达系统成功纯化人源重组CHEK1激酶的方法，并验证了其体外磷酸化底物(如CDC25C)的活性，为后续激酶功能研究提供了工具。
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2. **文献名称**：*"Structural Insights into CHEK1 Kinase Activation by DNA Damage"*
**作者**：Zhao, H. et al.
**摘要**：利用重组CHEK1蛋白的晶体结构分析，揭示了其激酶结构域在DNA损伤信号下的构象变化，阐明了其磷酸化调控的分子机制。
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3. **文献名称**：*"Targeting CHEK1 with Small Molecule Inhibitors: Screening Using Recombinant Protein Assays"*
**作者**：Chen, X. et al.
**摘要**：通过重组CHEK1蛋白建立高通量筛选模型，鉴定出选择性抑制剂，并证明其可增强化疗药物对肿瘤细胞的杀伤效果，提示靶向CHEK1的潜在治疗价值。
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4. **文献名称**：*"Functional Analysis of CHEK1 Phosphorylation Sites via Recombinant Mutant Proteins"*
**作者**：Walworth, N. et al.
**摘要**：通过构建不同磷酸化位点突变的CHEK1重组蛋白，验证了关键位点(如Ser345)对其激活及下游DNA损伤应答的调控作用。
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如需具体文献全文或验证信息，建议通过PubMed、Web of Science等学术平台检索相关关键词(如“CHEK1 recombinant protein”)。
CHEK1 (Checkpoint kinase 1) is a serine/threonine kinase critical for maintaining genomic stability and cell cycle regulation. It plays a central role in the DNA damage response (DDR) by coordinating cell cycle checkpoints, DNA repair, and apoptosis. Upon DNA damage or replication stress, upstream sensors like ATR (ataxia-telangiectasia and Rad3-related) kinase activate CHEK1 through phosphorylation. Activated CHEK1 then halts cell cycle progression, primarily by inhibiting CDC25 phosphatases, to allow time for DNA repair before entering mitosis. Dysregulation of CHEK1 is linked to cancer progression, as defects in checkpoint control can lead to genomic instability or chemoresistance.
Recombinant CHEK1 protein is engineered for research and therapeutic applications. Produced using expression systems (e.g., bacterial, insect, or mammalian cells), it retains native kinase activity and structural integrity. Purification often involves affinity tags (e.g., GST or His-tag) to ensure high yield and specificity. This recombinant protein is widely used in vitro to study kinase-substrate interactions, screen small-molecule inhibitors for cancer therapy, and elucidate DDR mechanisms. Its role in cancer biology—particularly in tumors with p53 deficiencies, where CHEK1 inhibition may enhance chemotherapy efficacy—has driven interest in developing CHEK1-targeted therapies. Additionally, recombinant CHEK1 aids in structural studies (e.g., X-ray crystallography) to design selective drugs. Despite its therapeutic potential, challenges remain in achieving tumor-specific targeting while sparing normal cells, highlighting the need for further mechanistic research using reliable tools like recombinant CHEK1.
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