| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MST4 |
| Uniprot No | Q9P289 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-416aa |
| 氨基酸序列 | MAHSPVAVQV PGMQNNIADP EELFTKLERI GKGSFGEVFK GIDNRTQQVV AIKIIDLEEA EDEIEDIQQE ITVLSQCDSS YVTKYYGSYL KGSKLWIIME YLGGGSALDL LRAGPFDEFQ IATMLKEILK GLDYLHSEKK IHRDIKAANV LLSEQGDVKL ADFGVAGQLT DTQIKRNTFV GTPFWMAPEV IQQSAYDSKA DIWSLGITAI ELAKGEPPNS DMHPMRVLFL IPKNNPPTLV GDFTKSFKEF IDACLNKDPS FRPTAKELLK HKFIVKNSKK TSYLTELIDR FKRWKAEGHS DDESDSEGSD SESTSRENNT HPEWSFTTVR KKPDPKKVQN GAEQDLVQTL SCLSMIITPA FAELKQQDEN NASRNQAIEE LEKSIAVAEA ACPGITDKMV KKLIEKFQKC SADESP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的3-4篇关于MST4重组蛋白研究的参考文献(注：文献为示例，实际文献请通过学术数据库检索)：
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1. **文献名称**：*"Recombinant MST4 kinase regulates cell polarity through phosphorylation of Ezrin"*
**作者**：Lin, F. et al.
**摘要**：研究利用重组MST4蛋白体外验证其对Ezrin蛋白的磷酸化作用，揭示其在细胞极性调控中的分子机制，为肿瘤转移研究提供依据。
2. **文献名称**：*"Structural insights into MST4 activation loop dynamics using crystallography"*
**作者**：Zhang, Y. et al.
**摘要**：通过重组表达人源MST4激酶结构域并解析其晶体结构，阐明其活性环构象变化对激酶活性的调控作用。
3. **文献名称**：*"MST4 recombinant protein suppresses hepatocellular carcinoma proliferation via Hippo pathway"*
**作者**：Wang, L. et al.
**摘要**：构建MST4重组蛋白并证明其过表达通过激活Hippo信号通路抑制肝癌细胞增殖，提示其潜在治疗价值。
4. **文献名称**：*"Optimizing expression and purification of MST4 in E. coli for high-throughput screening"*
**作者**：Kim, S. et al.
**摘要**：开发大肠杆菌系统中高效表达可溶性MST4重组蛋白的工艺，并应用于激酶抑制剂的药物筛选平台。
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建议通过 **PubMed** 或 **Web of Science** 检索关键词 "MST4 kinase"、"recombinant MST4" 获取真实文献。
MST4 (Mammalian Sterile20-like kinase 4), also known as STK26. is a serine/threonine kinase belonging to the STE20 family. It plays critical roles in cellular signaling pathways regulating cell polarity, migration, apoptosis, and stress responses. Structurally, MST4 contains an N-terminal catalytic kinase domain and a C-terminal regulatory region that facilitates protein-protein interactions. Its activity is tightly regulated by autophosphorylation and interaction with scaffolding proteins like STRAD and MO25. which modulate its subcellular localization and substrate specificity.
MST4 is implicated in diverse physiological and pathological processes. It interacts with the Golgi apparatus and participates in vesicle trafficking, influencing cell polarization and secretory pathways. In cancer biology, MST4 exhibits context-dependent roles, acting as either a tumor suppressor or promoter depending on tissue type. For instance, it suppresses hepatocellular carcinoma progression by inhibiting ERK signaling but promotes colorectal cancer growth via YAP activation. MST4 also regulates inflammatory responses by modulating macrophage activation and T-cell function, linking it to autoimmune diseases and metabolic disorders.
Recombinant MST4 protein is widely used in biochemical studies to dissect its kinase activity, substrate interactions, and regulatory mechanisms. Researchers employ expression systems (e.g., E. coli, insect cells) to produce purified MST4 for *in vitro* assays, structural analysis (e.g., X-ray crystallography), and drug discovery screens. Its recombinant form has helped identify small-molecule inhibitors targeting MST4 signaling in cancer and inflammatory conditions. Additionally, MST4’s role in cellular stress adaptation makes it a potential therapeutic target for diseases involving oxidative damage or metabolic dysregulation. Ongoing research continues to explore its complex interplay with other signaling hubs like AMPK and mTOR pathways.
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