| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAK3 |
| Uniprot No | O75914 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-559aa |
| 氨基酸序列 | MSDGLDNEEK PPAPPLRMNS NNRDSSALNH SSKPLPMAPE EKNKKARLRS IFPGGGDKTN KKKEKERPEI SLPSDFEHTI HVGFDAVTGE FTPDLYGSQM CPGKLPEGIP EQWARLLQTS NITKLEQKKN PQAVLDVLKF YDSKETVNNQ KYMSFTSGDK SAHGYIAAHP SSTKTASEPP LAPPVSEEED EEEEEEEDEN EPPPVIAPRP EHTKSIYTRS VVESIASPAV PNKEVTPPSA ENANSSTLYR NTDRQRKKSK MTDEEILEKL RSIVSVGDPK KKYTRFEKIG QGASGTVYTA LDIATGQEVA IKQMNLQQQP KKELIINEIL VMRENKNPNI VNYLDSYLVG DELWVVMEYL AGGSLTDVVT ETCMDEGQIA AVCRECLQAL DFLHSNQVIH RDIKSDNILL GMDGSVKLTD FGFCAQITPE QSKRSTMVGT PYWMAPEVVT RKAYGPKVDI WSLGIMAIEM VEGEPPYLNE NPLRALYLIA TNGTPELQNP ERLSAVFRDF LNRCLEMDVD RRGSAKELLQ HPFLKLAKPL SSLTPLIIAA KEAIKNSSR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PAK3重组蛋白的3篇参考文献(示例格式)：
1. **标题**：*Expression and purification of active recombinant PAK3 in Escherichia coli*
**作者**：Smith A, et al.
**摘要**：研究利用大肠杆菌表达系统成功表达PAK3重组蛋白，并通过亲和层析纯化获得高活性蛋白，验证其激酶活性及自磷酸化能力。
2. **标题**：*PAK3 interacts with β-Pix to regulate dendritic spine morphology through kinase-dependent pathways*
**作者**：Chen L, et al.
**摘要**：探讨PAK3重组蛋白与β-Pix的相互作用，证明其在神经元树突棘形态调控中的作用，通过体外激酶实验揭示其信号传导机制。
3. **标题**：*Biochemical characterization of PAK3 mutants associated with X-linked intellectual disability*
**作者**：Wang Y, et al.
**摘要**：分析PAK3致病突变体重组蛋白的酶动力学特性，发现突变导致激酶活性显著下降，提示其与智力障碍的分子关联。
(注：以上为模拟参考文献，实际文献需通过数据库如PubMed检索确认。)
PAK3 (p21-activated kinase 3) is a serine/threonine protein kinase belonging to the PAK family, which plays critical roles in regulating cytoskeletal dynamics, cell motility, and signaling pathways. It is activated by binding to small GTPases Cdc42 and Rac, leading to conformational changes that relieve autoinhibition and enable kinase activity. PAK3 is predominantly expressed in the brain, where it contributes to neuronal development, synaptic plasticity, and cognitive functions. Mutations in the PAK3 gene are linked to X-linked intellectual disability and neurodevelopmental disorders, highlighting its importance in neural circuitry and memory formation.
Recombinant PAK3 protein is produced using heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to study its biochemical properties, interactions, and downstream targets. Engineered with affinity tags (e.g., GST, His), the recombinant protein facilitates purification and application in enzymatic assays, structural studies, or inhibitor screening. Researchers utilize it to explore PAK3's role in diseases beyond neurology, including cancer, where aberrant PAK3 signaling promotes tumor invasion and metastasis. Its kinase domain and regulatory motifs are also targets for drug development, aiming to modulate pathways involved in pathological cell proliferation or neurodegeneration.
The recombinant form enables precise analysis of PAK3's activation mechanisms, post-translational modifications (e.g., phosphorylation), and crosstalk with other signaling networks like MAPK or PI3K/AKT. Studies using recombinant PAK3 have advanced understanding of how PAK dysregulation contributes to disease, offering a tool for therapeutic discovery and mechanistic validation in cellular and animal models.
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