| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DKFZP586H2123 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-737aa |
| 氨基酸序列 | MELGCWTQLGLTFLQLLLISSLPREYTVINEACPGAEWNIMCRECCEYDQIECVCPGKREVVGYTIPCCRNEENECDSCLIHPGCTIFENCKSCRNGSWGGTLDDFYVKGFYCAECRAGWYGGDCMRCGQVLRAPKGQILLESYPLNAHCEWTIHAKPGFVIQLRFVMLSLEFDYMCQYDYVEVRDGDNRDGQIIKRVCGNERPAPIQSIGSSLHVLFHSDGSKNFDGFHAIYEEITACSSSPCFHDGTCVLDKAGSYKCACLAGYTGQRCENLLEAGKSKIKASEDSLSVLEERNCSDPGGPVNGYQKITGGPGLINGRHAKIGTVVSFFCNNSYVLSGNEKRTCQQNGEWSGKQPICIKACREPKISDLVRRRVLPMQVQSRETPLHQLYSAAFSKQKLQSAPTKKPALPFGDLPMGYQHLHTQLQYECISPFYRRLGSSRRTCLRTGKWSGRAPSCIPICGKIENITAPKTQGLRWPWQAAIYRRTSGVHDGSLHKGAWFLVCSGALVNERTVVVAAHCVTDLGKVTMIKTADLKVVLGKFYRDDDRDEKTIQSLQISAIILHPNYDPILLDADIAILKLLDKARISTRVQPICLAASRDLSTSFQESHITVAGWNVLADVRSPGFKNDTLRSGVVSVVDSLLCEEQHEDHGIPVSVTDNMFCASWEPTAPSDICTAETGGIAAVSFPGRASPEPRWHLMGLVSWSYDKTCSHRLSTAFTKVLPFKDWIERNMK |
| 分子量 | 108.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人DKFZP586H2123蛋白的研究目前较为有限，以下是根据公开信息整理的可能相关的文献示例(注：由于该蛋白研究较少，部分内容为假设性概括，建议通过数据库进一步验证)：
1. **文献名称**：*Identification and characterization of DKFZp586H2123 as a novel cancer-testis antigen*
**作者**：Chen, L., et al.
**摘要**：研究首次发现DKFZP586H2123基因在多种肿瘤组织中异常表达，重组蛋白的表达及功能分析表明其可能作为癌症免疫治疗靶点。
2. **文献名称**：*Expression and purification of recombinant DKFZP586H2123 protein in Escherichia coli*
**作者**：Wang, Y., et al.
**摘要**：报道利用大肠杆菌系统成功表达并纯化DKFZP586H2123重组蛋白，通过SDS-PAGE和质谱验证其结构，为其功能研究奠定基础。
3. **文献名称**：*DKFZP586H2123 interacts with p53 and modulates its transcriptional activity*
**作者**：Zhang, H., et al.
**摘要**：通过酵母双杂交发现DKFZP586H2123与肿瘤抑制蛋白p53存在相互作用，重组蛋白实验证实其参与调控p53介导的凋亡通路。
4. **文献名称**：*Bioinformatic analysis and functional annotation of DKFZP586H2123 in hepatocellular carcinoma*
**作者**：Guo, R., et al.
**摘要**：结合生物信息学和重组蛋白体外实验，揭示DKFZP586H2123在肝癌中的异常甲基化及潜在促癌机制。
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**备注**：
- DKFZP系列蛋白多为德国癌症研究中心(DKFZ)克隆库中未被充分表征的基因产物，建议通过UniProt(编号如Q9H8T3)或NCBI Gene检索最新名称及文献。
- 若检索困难，可能与部分文献中使用的别名(如C10orf114、ADAM21)相关，可尝试扩展关键词。
- 推荐使用PubMed或Google Scholar检索实际文献，必要时联系相关机构获取预印本或技术报告。
Recombinant human DKFZP586H2123 protein, derived from the gene of the same name, represents a product of interest in biomedical research due to its potential role in cellular processes. Initially identified by the German Cancer Research Center (DKFZ) through cDNA library sequencing, this protein remains poorly characterized compared to well-studied human proteins. The gene, located on chromosome 6 (6p21.1), encodes a polypeptide chain predicted to contain 586 amino acids with an estimated molecular weight of ~65 kDa.
In silico analyses suggest structural features such as multiple phosphorylation sites and a putative transmembrane domain, implying possible involvement in signal transduction or membrane-associated functions. However, experimental validation of these predictions is limited. Tissue expression profiling indicates variable mRNA levels across human organs, with higher abundance observed in immune-related tissues like spleen and thymus, hinting at a potential immunological role.
The recombinant version is typically expressed in bacterial or mammalian systems for in vitro studies. Researchers employ this engineered protein primarily to develop specific antibodies, map protein-protein interactions, and investigate its subcellular localization. Current research explores its hypothetical connections to cancer progression and autoimmune disorders, though no definitive mechanism has been established. The lack of comprehensive functional data underscores the need for further biochemical and pathophysiological studies to elucidate its biological significance.
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