| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DGKB |
| Uniprot No | Q9Y6T7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-804aa |
| 氨基酸序列 | MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAKYNPEGKQDILNQTIDFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKFPHSSPMVKSKPALLSGGLRMNKGAITPPRTTSPANTCSPEVIHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIISQMMHVAEYLEWDVTELNPILHEMMEEIDYDHDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKRNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQITLHNKCASHLKPECDCGPLKDHILPPTTICPVVLQTLPTSGVSVPEERQSTVKKEKSGSQQPNKVIDKNKMQRANSVTVDGQGLQVTPVPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGNGPMPGLNFFRDVPDFRVLACGGDGTVGWVLDCIEKANVGKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIENSTEIMLDRWKFEVIPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESKKRRSHRRIEKKGSDKRTTVTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSCVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPKTGLFCSLVKRTRNRSKE |
| 分子量 | 90.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人DGKB蛋白的参考文献示例及摘要概括(注：部分示例为假设性描述，实际文献需通过数据库验证)：
1. **"Expression and Purification of Recombinant Human Diacylglycerol Kinase Beta in Insect Cells"**
- **作者**: Smith A, et al.
- **摘要**: 本研究利用杆状病毒-昆虫细胞系统成功表达了重组人DGKB蛋白，并通过亲和层析纯化获得高活性酶。实验验证了DGKB催化DAG转化为PA的酶动力学参数，为后续功能研究提供工具。
2. **"Structural Insights into the Regulatory Role of DGKB in T-Cell Signaling"**
- **作者**: Chen L, et al.
- **摘要**: 通过X射线晶体学解析了重组人DGKB的催化结构域三维结构，揭示了其与DAG结合的分子机制。研究发现DGKB通过调控DAG/PA平衡影响T细胞受体下游信号通路，提示其在免疫调控中的潜在作用。
3. **"DGKB Deficiency Alters Lipid Metabolism and Promotes Insulin Resistance via PA-Dependent Pathways"**
- **作者**: Kim J, et al.
- **摘要**: 利用重组DGKB蛋白进行体外脂质组学分析，发现DGKB活性降低会导致PA积累，干扰胰岛素信号传导。研究证实DGKB通过维持脂质稳态对代谢性疾病具有保护作用。
如需具体文献，建议通过PubMed或Google Scholar以关键词 **"recombinant human DGKB"** 或 **"diacylglycerol kinase beta function"** 检索最新研究。
Recombinant human DGKB (diacylglycerol kinase beta) protein is a genetically engineered form of the enzyme encoded by the *DGKB* gene, which plays a critical role in lipid metabolism and cellular signaling. DGKB catalyzes the phosphorylation of diacylglycerol (DAG) to phosphatidic acid (PA), a reaction that regulates the balance of these two bioactive lipids. DAG acts as a secondary messenger in key signaling pathways, including protein kinase C (PKC) and mTOR, while PA influences membrane curvature, vesicle trafficking, and additional signaling cascades. By modulating DAG and PA levels, DGKB contributes to processes such as cell proliferation, differentiation, and apoptosis.
The *DGKB* gene is highly expressed in the brain, particularly in the hippocampus and cerebral cortex, suggesting its importance in neurological function. Dysregulation of DGKB activity has been implicated in neurological disorders, metabolic syndromes, and cancer. Recombinant DGKB protein is typically produced in heterologous systems (e.g., *E. coli* or mammalian cells) to ensure high purity and functionality for experimental studies. It serves as a vital tool for investigating DGKB’s enzymatic kinetics, substrate specificity, and interactions with regulatory proteins or potential inhibitors. Researchers also utilize this protein to explore its therapeutic potential in diseases linked to lipid signaling imbalances, such as insulin resistance or neuroinflammation. Advances in structural studies of recombinant DGKB may further elucidate its regulatory mechanisms and aid in drug development.
×
