| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STK10 |
| Uniprot No | O94804 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-968aa |
| 氨基酸序列 | MAFANFRRILRLSTFEKRKSREYEHVRRDLDPNEVWEIVGELGDGAFGKV YKAKNKETGALAAAKVIETKSEEELEDYIVEIEILATCDHPYIVKLLGAY YHDGKLWIMIEFCPGGAVDAIMLELDRGLTEPQIQVVCRQMLEALNFLHS KRIIHRDLKAGNVLMTLEGDIRLADFGVSAKNLKTLQKRDSFIGTPYWMA PEVVMCETMKDTPYDYKADIWSLGITLIEMAQIEPPHHELNPMRVLLKIA KSDPPTLLTPSKWSVEFRDFLKIALDKNPETRPSAAQLLEHPFVSSITSN KALRELVAEAKAEVMEEIEDGRDEGEEEDAVDAASTLENHTQNSSEVSPP SLNADKPLEESPSTPLAPSQSQDSVNEPCSQPSGDRSLQTTSPPVVAPGN ENGLAVPVPLRKSRPVSMDARIQVAQEKQVAEQGGDLSPAANRSQKASQS RPNSSALETLGGEKLANGSLEPPAQAAPGPSKRDSDCSSLCTSESMDYGT NLSTDLSLNKEMGSLSIKDPKLYKKTLKRTRKFVVDGVEVSITTSKIISE DEKKDEEMRFLRRQELRELRLLQKEEHRNQTQLSNKHELQLEQMHKRFEQ EINAKKKFFDTELENLERQQKQQVEKMEQDHAVRRREEARRIRLEQDRDY TRFQEQLKLMKKEVKNEVEKLPRQQRKESMKQKMEEHTQKKQLLDRDFVA KQKEDLELAMKRLTTDNRREICDKERECLMKKQELLRDREAALWEMEEHQ LQERHQLVKQQLKDQYFLQRHELLRKHEKEREQMQRYNQRMIEQLKVRQQ QEKARLPKIQRSEGKTRMAMYKKSLHINGGGSAAEQREKIKQFSQQEEKR QKSERLQQQQKHENQMRDMLAQCESNMSELQQLQNEKCHLLVEHETQKLK ALDESHNQNLKEWRDKLRPRKKAL |
| 预测分子量 | 138 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STK10重组蛋白的3篇参考文献示例(注：文献信息为模拟概括，实际文献需根据具体数据库检索)：
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1. **文献名称**: *"Expression and purification of recombinant human STK10 kinase domain in Escherichia coli for structural studies"*
**作者**: Smith A, et al.
**摘要**: 本研究成功构建了人源STK10激酶结构域的重组表达系统，利用大肠杆菌表达并优化纯化条件，获得了高纯度蛋白。通过X射线晶体学分析了其三维结构，为STK10的酶活性机制研究奠定了基础。
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2. **文献名称**: *"Functional characterization of STK10 in T-cell signaling using recombinant protein assays"*
**作者**: Lee B, et al.
**摘要**: 作者在昆虫细胞中表达了重组STK10蛋白，并验证其体外激酶活性。实验表明STK10通过磷酸化下游靶点LATS1参与调控T细胞迁移，揭示了其在免疫调节中的潜在作用。
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3. **文献名称**: *"STK10 interacts with ERM proteins via its FERM domain: Insights from recombinant protein binding assays"*
**作者**: Zhang Y, et al.
**摘要**: 该研究通过重组STK10蛋白与Ezrin/Radixin/Moesin(ERM)蛋白的体外结合实验，证实其FERM结构域介导相互作用，提示STK10可能通过调控细胞骨架动力学影响肿瘤细胞侵袭。
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如需具体文献，建议通过PubMed或Web of Science检索关键词“STK10 recombinant protein”获取最新研究。
**Background of STK10 Recombinant Protein**
STK10 (Serine/Threonine Kinase 10), also known as LOK or ARAK, is a member of the STE20 family of protein kinases. It plays a critical role in regulating cellular processes such as cytoskeletal organization, cell migration, and immune response. Structurally, STK10 contains an N-terminal kinase domain and a C-terminal regulatory region, which mediates interactions with signaling adaptors or substrates. Its kinase activity is tightly controlled through phosphorylation and protein-protein interactions, particularly with the downstream effector proteins LATS1/2. linking it to the Hippo signaling pathway.
STK10 is highly expressed in immune cells, notably T lymphocytes, where it modulates cytoskeletal dynamics and cell polarity. Studies suggest its involvement in maintaining immune homeostasis, as dysregulation of STK10 has been associated with autoimmune disorders and lymphoproliferative diseases. For instance, mutations or altered expression of STK10 may impair T-cell function, contributing to pathologies like lymphoma or systemic lupus erythematosus (SLE).
Recombinant STK10 protein is produced using expression systems (e.g., *E. coli*, mammalian cells) to ensure proper folding and enzymatic activity. It serves as a vital tool for *in vitro* studies, enabling researchers to investigate kinase-substrate relationships, screen for inhibitors, or map signaling networks. Its applications extend to drug discovery, particularly in targeting immune-related diseases or cancers driven by aberrant Hippo pathway activity. Additionally, recombinant STK10 aids in structural studies, providing insights into kinase activation mechanisms and guiding the design of therapeutic molecules.
Overall, STK10 recombinant protein is a valuable resource for unraveling the biological and pathological roles of this kinase, with translational potential in immunology and oncology research.
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