| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRK6 |
| Uniprot No | P43250 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-576aa |
| 氨基酸序列 | MELENIVANT VLLKAREGGG GNRKGKSKKW RQMLQFPHIS QCEELRLSLE RDYHSLCERQ PIGRLLFREF CATRPELSRC VAFLDGVAEY EVTPDDKRKA CGRQLTQNFL SHTGPDLIPE VPRQLVTNCT QRLEQGPCKD LFQELTRLTH EYLSVAPFAD YLDSIYFNRF LQWKWLERQP VTKNTFRQYR VLGKGGFGEV CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKQILEK VNSRFVVSLA YAYETKDALC LVLTLMNGGD LKFHIYHMGQ AGFPEARAVF YAAEICCGLE DLHRERIVYR DLKPENILLD DHGHIRISDL GLAVHVPEGQ TIKGRVGTVG YMAPEVVKNE RYTFSPDWWA LGCLLYEMIA GQSPFQQRKK KIKREEVERL VKEVPEEYSE RFSPQARSLC SQLLCKDPAE RLGCRGGSAR EVKEHPLFKK LNFKRLGAGM LEPPFKPDPQ AIYCKDVLDI EQFSTVKGVE LEPTDQDFYQ KFATGSVPIP WQNEMVETEC FQELNVFGLD GSVPPDLDWK GQPPAPPKKG LLQRLFSRQD CCGNCSDSEE ELPTRL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GRK6重组蛋白的模拟参考文献示例(建议通过学术数据库验证具体文献)：
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1. **文献名称**: "Expression and Purification of Recombinant GRK6 in Escherichia coli for Functional Studies"
**作者**: Smith A, et al.
**摘要**: 本研究报道了在大肠杆菌中高效表达人源GRK6重组蛋白的优化方法，通过亲和层析和尺寸排阻色谱纯化获得高纯度蛋白，并验证其激酶活性。结果显示重组GRK6能够磷酸化G蛋白偶联受体(GPCR)，为体外研究其调控机制提供了工具。
2. **文献名称**: "Structural Insights into GRK6 Activation Mechanism via Cryo-EM Analysis"
**作者**: Johnson R, et al.
**摘要**: 通过冷冻电镜技术解析了GRK6重组蛋白与β2肾上腺素受体复合物的三维结构，揭示了GRK6的构象变化及其与受体结合的分子细节，为开发靶向GRK6的抑制剂提供了结构基础。
3. **文献名称**: "GRK6-Mediated Phosphorylation in Immune Cell Signaling: Role of Recombinant Protein Assays"
**作者**: Lee H, et al.
**摘要**: 利用重组GRK6蛋白研究其在T细胞信号通路中的作用，发现GRK6通过磷酸化特定免疫受体调节炎症反应。实验表明，重组GRK6的酶活性能被小分子化合物选择性抑制，提示其作为免疫治疗靶点的潜力。
4. **文献名称**: "Kinetic Characterization of Recombinant GRK6 and Its Variants in Neurodegenerative Disease Models"
**作者**: Garcia M, et al.
**摘要**: 通过体外动力学实验比较了野生型与突变型GRK6重组蛋白的活性差异，发现某些帕金森病相关突变会显著降低其磷酸化能力，为疾病机制研究提供了生化证据。
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**注意**：以上文献为模拟示例，实际引用时请通过PubMed、Web of Science等平台检索真实文献。建议使用关键词“GRK6 recombinant protein expression”或“GRK6 kinase activity”进行精准查询。
GRK6 (G protein-coupled receptor kinase 6) is a member of the GRK family, a group of serine/threonine kinases that regulate the activity of G protein-coupled receptors (GPCRs) by phosphorylating their intracellular domains upon receptor activation. This phosphorylation promotes the binding of β-arrestin, leading to receptor desensitization, internalization, and downstream signaling modulation. GRK6 is broadly expressed in immune cells, the cardiovascular system, and the central nervous system, playing critical roles in physiological processes such as immune response, neurotransmitter signaling, and cardiac function. Dysregulation of GRK6 has been implicated in various pathologies, including inflammatory disorders, cancer metastasis, and cardiovascular diseases.
Recombinant GRK6 protein is engineered using heterologous expression systems (e.g., bacterial, insect, or mammalian cells) to produce purified, functional kinase for research and therapeutic development. It typically retains catalytic activity and structural features of native GRK6. including its N-terminal RGS (Regulator of G protein Signaling) homology domain and C-terminal kinase domain. Researchers utilize recombinant GRK6 to study GPCR signaling mechanisms, screen for kinase inhibitors or modulators, and investigate its role in disease pathways. Its applications extend to structural studies (e.g., X-ray crystallography) to elucidate molecular interactions with receptors or drugs. In drug discovery, GRK6 is a potential target for immune-related therapies, as its inhibition has shown promise in modulating T-cell activation and cytokine production in autoimmune conditions. The development of recombinant GRK6 has significantly advanced mechanistic studies and translational research aimed at targeting GPCR-related signaling networks.
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