| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FES |
| Uniprot No | P07332 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-822aa |
| 氨基酸序列 | GFSSELCSPQGHGVLQQMQEAELRLLEGMRKWMAQRVKSDREYAGLLHHM SLQDSGGQSRAISPDSPISQSWAEITSQTEGLSRLLRQHAEDLNSGPLSK LSLLIRERQQLRKTYSEQWQQLQQELTKTHSQDIEKLKSQYRALARDSAQ AKRKYQEASKDKDRDKAKDKYVRSLWKLFAHHNRYVLGVRAAQLHHQHHH QLLLPGLLRSLQDLHEEMACILKEILQEYLEISSLVQDEVVAIHREMAAA AARIQPEAEYQGFLRQYGSAPDVPPCVTFDESLLEEGEPLEPGELQLNE LTVESVQHTLTSVTDELAVATEMVFRRQEMVTQLQQELRNEEENTHPRE RVQLLGKRQVLQEALQGLQVALCSQAKLQAQQELLQTKLEHLGPGEPPPV LLLQDDRHSTSSSEQEREGGR TPTLEILKSHISGIFRPKFSLPPPLQL IPEVQKPLHEQLWYHGAIPRAEVAELLVHSGDFLVRESQGKQEYVLSVLW DGLPRHFIIQSLDNLYRLEGEGFPSIPLLIDHLLSTQQPLTKKSGVVLHR AVPKDKWVLNHEDLVLGEQIGRGNFGEVFSGRLRADNTLVAVKSCRETLP PDLKAKFLQEARILKQYSHPNIVRLIGVCTQKQPIYIVMELVQGGDFLTF LRTEGARLRVKTLLQMVGDAAAGMEYLESKCCIHRDLAARNCLVTEKNVL KISDFGMSREEADGVYAASGGLRQVPVKWTAPEALNYGRYSSESDVWSFG ILLWETFSLGASPYPNLSNQQTREFVEKGGRLPCPELCPDAVFRLMEQCW AYEPGQRPSFSTIYQELQSIRKRHR |
| 预测分子量 | 95 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FES重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *"Functional characterization of the c-Fes protein-tyrosine kinase"*
**作者**: Greer, P. et al.
**摘要**: 研究通过重组c-Fes蛋白在哺乳动物细胞中的表达，揭示了其酪氨酸激酶活性对髓系细胞分化的调控作用，并发现其SH2结构域在底物识别中的关键功能。
2. **文献名称**: *"Expression and purification of active FES tyrosine kinase in Escherichia coli"*
**作者**: Kim, J.H. & Lee, S.T.
**摘要**: 开发了在大肠杆菌中高效表达可溶性重组FES激酶的方法，纯化后的蛋白在体外表现出磷酸化底物及自磷酸化活性，为激酶抑制剂筛选提供工具。
3. **文献名称**: *"The Fes kinase regulates mast cell adhesion and migration"*
**作者**: Voisset, E. et al.
**摘要**: 利用重组FES蛋白及基因敲除模型，证明FES通过磷酸化黏着斑相关蛋白调控肥大细胞迁移，提示其在炎症和肿瘤转移中的潜在作用。
(注：以上文献信息为示例性概括，实际引用时需核对原文准确性。)
FES (Feline Sarcoma Oncogene) recombinant proteins are derived from the c-fes/fps proto-oncogene, which encodes a non-receptor tyrosine kinase involved in cellular signaling pathways. Initially identified in feline sarcoma retroviruses, the FES kinase plays regulatory roles in hematopoiesis, immune response, and cell differentiation. Structurally, FES contains an N-terminal FCH (FES/FER/CIP4 homology) domain, a central SH2 (Src homology 2) domain for phosphotyrosine-mediated protein interactions, and a C-terminal kinase domain. Its activation regulates downstream targets like STAT3 and PI3K/AKT pathways, influencing cell proliferation, survival, and cytoskeletal dynamics.
Recombinant FES proteins are generated using biotechnological methods, often expressed in bacterial or mammalian systems for functional studies. These proteins retain kinase activity and binding properties, enabling researchers to investigate FES-mediated signaling mechanisms in vitro. Applications include kinase activity assays, protein interaction studies, and screening for inhibitors in cancer research. Dysregulation of FES is linked to leukemia, breast cancer, and inflammatory diseases, making its recombinant forms valuable for dissecting oncogenic mutations or designing targeted therapies. Recent studies also explore FES roles in macrophage polarization and endothelial cell functions, highlighting its therapeutic potential beyond oncology. Optimized recombinant FES variants with enhanced stability or tagged epitopes (e.g., His-tag, GFP) further facilitate structural analyses and high-throughput drug discovery.
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