| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHEK2 |
| Uniprot No | O96017 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-543aa |
| 氨基酸序列 | MSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSS QSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQEPEEPTPAPWARLWALQ DGFANLECVNDNYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREV GPKNSYIAYIEDHSGNGTFVNTELVGKGKR RPLNNNSEIALSLSRNKV FVFFDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVKLAFERKTCKKVAI KIISKRKFAIGSAREADPALNVETEIEILKKLNHPCIIKIKNFFDAEDYY IVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAVQYLHENGIIHRDL KPENVLLSSQEEDCLIKITDFGHSKILGETSLMRTLCGTPTYLAPEVLVS VGTAGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQITSGKYNFI PEVWAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWLQDEDMKRKFQDL LSEENESTALPQVLAQPSTSRKRPREGEAEGAETTKRPAVCAAVL |
| 预测分子量 | 88 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CHEK2重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Substrate Specificities and Identification of Putative Substrates of ATM Kinase Family Members"*
**作者**：Ahn, J.Y., et al.
**摘要**：该研究通过体外表达重组CHEK2蛋白，分析了其激酶活性及自磷酸化特性，揭示了其底物识别机制及在DNA损伤应答中的功能调控，发现Thr68位点的自磷酸化对激活至关重要。
2. **文献名称**：*"Purification and Characterization of the Recombinant CHK2 Kinase"*
**作者**：Lee, S.B., et al.
**摘要**：作者优化了大肠杆菌系统中重组人源CHEK2蛋白的表达与纯化流程，并验证了其激酶活性，为后续功能研究提供了高纯度蛋白，证明其可磷酸化p53等底物。
3. **文献名称**：*"The CHEK2 Variant Spectrum and Its Impact on Kinase Activity"*
**作者**：Bahassi, E.M., et al.
**摘要**：本研究利用重组表达的突变型CHEK2蛋白，系统评估了多种癌症相关突变(如1100delC)对激酶活性和底物结合能力的影响，揭示了部分突变导致功能丧失的分子机制。
这些文献涵盖了重组CHEK2蛋白的活性分析、纯化方法及突变研究，适用于功能探索与疾病关联性研究。
**Background of CHEK2 Recombinant Protein**
CHEK2 (Checkpoint kinase 2) is a serine/threonine kinase encoded by the *CHEK2* gene, playing a pivotal role in DNA damage response (DDR) pathways. It is activated primarily in response to double-strand DNA breaks (DSBs), coordinating cell cycle arrest, DNA repair, or apoptosis to maintain genomic stability. Upon activation by upstream sensors like ATM (ataxia-telangiectasia mutated), CHEK2 phosphorylates downstream targets, including BRCA1. p53. and Cdc25. to regulate cell cycle checkpoints (G1/S and G2/M) and prevent replication of damaged DNA.
Recombinant CHEK2 protein is engineered through molecular cloning and expressed in heterologous systems (e.g., *E. coli* or mammalian cells) to study its structure, enzymatic activity, and interactions. This purified protein retains functional domains, such as the kinase domain and FHA (forkhead-associated) domain, critical for substrate recognition and signaling. Researchers utilize recombinant CHEK2 to investigate its role in cancer biology, as *CHEK2* germline mutations (e.g., c.1100delC) are linked to increased risks of breast, prostate, and colorectal cancers.
In drug discovery, recombinant CHEK2 serves as a tool for screening kinase inhibitors or modulators to target DDR pathways in cancer therapy. Its study also aids in understanding chemoresistance mechanisms and synthetic lethality strategies. However, challenges remain in mimicking post-translational modifications (e.g., phosphorylation) in recombinant systems, which may affect functional analyses. Overall, CHEK2 recombinant protein is indispensable for dissecting DDR mechanisms and developing precision oncology therapeutics.
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