| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS6KA6 |
| Uniprot No | Q9UK32 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-745aa |
| 氨基酸序列 | MLPFAPQDEP WDREMEVFSG GGASSGEVNG LKMVDEPMEE GEADSCHDEG VVKEIPITHH VKEGYEKADP AQFELLKVLG QGSFGKVFLV RKKTGPDAGQ LYAMKVLKKA SLKVRDRVRT KMERDILVEV NHPFIVKLHY AFQTEGKLYL ILDFLRGGDV FTRLSKEVLF TEEDVKFYLA ELALALDHLH QLGIVYRDLK PENILLDEIG HIKLTDFGLS KESVDQEKKA YSFCGTVEYM APEVVNRRGH SQSADWWSYG VLMFEMLTGT LPFQGKDRNE TMNMILKAKL GMPQFLSAEA QSLLRMLFKR NPANRLGSEG VEEIKRHLFF ANIDWDKLYK REVQPPFKPA SGKPDDTFCF DPEFTAKTPK DSPGLPASAN AHQLFKGFSF VATSIAEEYK ITPITSANVL PIVQINGNAA QFGEVYELKE DIGVGSYSVC KRCIHATTNM EFAVKIIDKS KRDPSEEIEI LMRYGQHPNI ITLKDVFDDG RYVYLVTDLM KGGELLDRIL KQKCFSEREA SDILYVISKT VDYLHCQGVV HRDLKPSNIL YMDESASADS IRICDFGFAK QLRGENGLLL TPCYTANFVA PEVLMQQGYD AACDIWSLGV LFYTMLAGYT PFANGPNDTP EEILLRIGNG KFSLSGGNWD NISDGAKDLL SHMLHMDPHQ RYTAEQILKH SWITHRDQLP NDQPKRNDVS HVVKGAMVAT YSALTHKTFQ PVLEPVAASS LAQRRSMKKR TSTGL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS6KA6重组蛋白的参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库验证)：
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1. **文献名称**：*"Expression and Functional Characterization of Recombinant RPS6KA6 in Mammalian Cells"*
**作者**：Zhang Y, et al.
**摘要**：本研究成功在HEK293细胞中表达并纯化了重组RPS6KA6蛋白，证实其具有MAPK信号通路依赖的激酶活性，并发现其通过磷酸化下游转录因子调控细胞应激反应。
2. **文献名称**：*"Structural Basis of RPS6KA6 Activation by ERK Signaling"*
**作者**：Thompson R, et al.
**摘要**：通过晶体结构分析和体外激酶实验，揭示了ERK介导的重组RPS6KA6激活机制，阐明了其C端结构域在底物识别中的关键作用。
3. **文献名称**：*"RPS6KA6 Recombinant Protein as a Potential Therapeutic Target in Colorectal Cancer"*
**作者**：Gupta S, et al.
**摘要**：利用重组RPS6KA6蛋白进行功能研究，发现其异常表达与结直肠癌细胞增殖和化疗耐药性相关，提示其作为治疗靶点的潜力。
4. **文献名称**：*"In Vitro Kinase Activity Profiling of Human RSK Family Members: Insights into RPS6KA6 Substrate Specificity"*
**作者**：Müller P, et al.
**摘要**：比较了RSK家族成员(包括重组RPS6KA6)的激酶活性，鉴定出多个新型底物，并揭示了RPS6KA6在细胞周期调控中的独特功能。
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建议通过PubMed、Google Scholar等平台以“RPS6KA6 recombinant protein”“RPS6KA6 kinase activity”为关键词检索最新文献，或查阅《Journal of Biological Chemistry》《Cell Signaling》等期刊的相关研究。
The RPS6KA6 recombinant protein, also known as ribosomal protein S6 kinase A6. belongs to the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This protein is encoded by the *RPS6KA6* gene and plays a role in cellular signaling pathways, particularly those mediated by the mitogen-activated protein kinase (MAPK) cascade. RSK family members are activated via phosphorylation by extracellular signal-regulated kinases (ERKs), linking extracellular signals to downstream targets involved in cell growth, survival, and differentiation. RPS6KA6 contains two functional kinase domains and a regulatory linker region, enabling its participation in diverse cellular processes, including transcriptional regulation and stress responses.
Recombinant RPS6KA6 is engineered in vitro using expression systems like *E. coli* or mammalian cell lines to produce purified, biologically active protein for research. Its production often involves affinity tags (e.g., His-tag) for simplified purification and detection. Researchers utilize this recombinant protein to study kinase-substrate interactions, signaling mechanisms, and pathway crosstalk in diseases such as cancer, neurodegenerative disorders, and metabolic syndromes. Dysregulation of RPS6KA6 has been implicated in tumor progression and resistance to therapies, making it a potential target for drug development. Additionally, its role in neuronal survival pathways highlights relevance in neurological research. The availability of recombinant RPS6KA6 facilitates high-throughput screening for inhibitors/activators and mechanistic studies, advancing both basic science and therapeutic innovation.
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