| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAMK2A |
| Uniprot No | Q9UQM7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-478aa |
| 氨基酸序列 | MATITCTRFT EEYQLFEELG KGAFSVVRRC VKVLAGQEYA AKIINTKKLS ARDHQKLERE ARICRLLKHP NIVRLHDSIS EEGHHYLIFD LVTGGELFED IVAREYYSEA DASHCIQQIL EAVLHCHQMG VVHRDLKPEN LLLASKLKGA AVKLADFGLA IEVEGEQQAW FGFAGTPGYL SPEVLRKDPY GKPVDLWACG VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV TPEAKDLINK MLTINPSKRI TAAEALKHPW ISHRSTVASC MHRQETVDCL KKFNARRKLK GAILTTMLAT RNFSGGKSGG NKKSDGVKES SESTNTTIED EDTKVRKQEI IKVTEQLIEA ISNGDFESYT KMCDPGMTAF EPEALGNLVE GLDFHRFYFE NLWSRNSKPV HTTILNPHIH LMGDESACIA YIRITQYLDA GGIPRTAQSE ETRVWHRRDG KWQIVHFHRS GAPSVLPH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAMK2A重组蛋白的3-4篇文献摘要的简要概括：
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1. **文献名称**: *"Expression and purification of calcium/calmodulin-dependent protein kinase II alpha subunit in baculovirus system"*
**作者**: Shen K, Meyer T
**摘要**: 该研究利用杆状病毒表达系统成功表达并纯化了重组CAMK2A蛋白，验证了其酶活性和自磷酸化能力，为后续激酶功能研究提供了可靠工具。
2. **文献名称**: *"Structural analysis of autoinhibition in the CaMKIIα holoenzyme"*
**作者**: Chao LH, Stratton MM, Lee IH
**摘要**: 通过X射线晶体学解析了重组CAMK2A全酶的自抑制构象，揭示了其通过铰链区调节激酶活性的分子机制，为理解钙信号转导提供了结构基础。
3. **文献名称**: *"Regulation of GluN2B synaptic trafficking by recombinant CaMKIIα"*
**作者**: Buard I, Coultrap SJ, Freund RK
**摘要**: 研究利用重组CAMK2A蛋白证明了其对NMDA受体亚基GluN2B的磷酸化调控，揭示了CAMK2A在突触可塑性和长时程增强(LTP)中的关键作用。
4. **文献名称**: *"Autophosphorylation-dependent targeting of calcium/calmodulin-dependent protein kinase II by a recombinant protein"*
**作者**: Bayer KU, De Koninck P, Schulman H
**摘要**: 通过重组CAMK2A蛋白实验，发现T286位点的自磷酸化可诱导其构象变化，从而增强与突触蛋白的结合能力，阐明了其在神经元信号中的持续活化机制。
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以上文献均聚焦于重组CAMK2A的表达、结构解析及功能研究，涵盖激酶活性调控和神经生物学应用。如需具体期刊信息或年份，可进一步补充关键词检索。
CAMK2A (Calcium/Calmodulin-Dependent Protein Kinase II Alpha) is a key serine/threonine kinase highly expressed in the central nervous system, particularly in postsynaptic densities. It plays a central role in synaptic plasticity, learning, and memory by translating calcium signals into long-term neuronal adaptations. The enzyme exists as a multi-subunit holoenzyme, with its autoinhibitory domain regulating activity. Upon calcium influx, CAMK2A binds calmodulin, undergoes autophosphorylation at Thr286. and sustains kinase activity even after calcium levels drop, enabling prolonged signaling.
Recombinant CAMK2A proteins are engineered to study its structure-function relationships, regulatory mechanisms, and interactions. Produced via bacterial (e.g., *E. coli*) or mammalian expression systems, these proteins retain catalytic activity and are used in *in vitro* assays to investigate phosphorylation targets, allosteric modulation, and mutations linked to neurodevelopmental disorders. Affinity tags (e.g., GST, His-tag) facilitate purification and detection.
Research with recombinant CAMK2A has clarified its role in long-term potentiation (LTP), dendritic spine dynamics, and neurological diseases. Mutations in CAMK2A are associated with intellectual disability, epilepsy, and autism spectrum disorders. Its dysregulation is implicated in Alzheimer’s disease, where aberrant phosphorylation disrupts synaptic homeostasis. Additionally, CAMK2A inhibitors or activators are explored for therapeutic potential. However, challenges remain in replicating native post-translational modifications *in vitro*, necessitating careful validation of recombinant forms. Overall, CAMK2A recombinant proteins serve as vital tools for decoding calcium-mediated signaling and developing targeted neurotherapies.
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