| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CWF19L2 |
| Uniprot No | Q2TBE0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-740aa |
| 氨基酸序列 | MTVDFMSVKTVSSSSLKAEKETMRKIEQEKNQALEQSKLMERELNPYWKDGGTGLPPEDCSVSSITKVSVVEDGGLSWLRKSYLRMKEQAEKQSRNFEDIVAERYGSMEIFQSKLEDAEKAASTKEDYRRERWRKPTYSDKAQNCQESRESDLVKYGNSSRDRYATTDTAKNSNNEKFIGDEKDKRPGSLETCRRESNPRQNQEFSFGNLRAKFLRPSDDEELSFHSKGRKFEPLSSSSALVAQGSLCSGFRKPTKNSEERLTSWSRSDGRGDKKHSNQKPSETSTDEHQHVPEDPREKSQDEVLRDDPPKKEHLRDTKSTFAGSPERESIHILSVDEKNKLGAKIIKAEMMGNMELAEQLKVQLEKANKFKETITQIPKKSGVENEDQQEVILVRTDQSGRVWPVNTPGKSLESQGGRRKRQMVSTHEERERVRYFHDDDNLSLNDLVKNEKMGTAENQNKLFMRMASKFMGKTDGDYYTLDDMFVSKAAERERLGEEEENQRKKAIAEHRSLAAQMEKCLYCFDSSQFPKHLIVAIGVKVYLCLPNVRSLTEGHCLIVPLQHHRAATLLDEDIWEEIQMFRKSLVKMFEDKGLDCIFLETNMSMKKQYHMVYECIPLPKEVGDMAPIYFKKAIMESDEEWSMNKKLIDLSSKDIRKSVPRGLPYFSVDFGLHGGFAHVIEDQHKFPHYFGKEIIGGMLDIEPRLWRKGIRESFEDQRKKALQFAQWWKPYDFTKSKNY |
| 分子量 | 112.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人CWF19L2蛋白的参考文献示例(注：以下内容为模拟虚构，仅作格式参考)：
1. **《Functional characterization of recombinant human CWF19L2 protein in cell cycle regulation》**
*Authors: Zhang Y., Li X., Wang H.*
摘要：本研究首次在大肠杆菌系统中成功表达重组人CWF19L2蛋白，并验证其通过泛素化途径调控G2/M期检查点的功能，揭示其与CDC25C磷酸酶的相互作用。
2. **《CWF19L2 recombinant protein inhibits tumor growth in xenograft models》**
*Authors: Tanaka S., Gupta R., Müller J.*
摘要：通过哺乳动物细胞表达系统制备高纯度CWF19L2重组蛋白，实验表明其通过激活JNK信号通路抑制结直肠癌小鼠模型的肿瘤增殖，提出其潜在抗癌治疗价值。
3. **《Structural analysis and proteomic profiling of CWF19L2 in neurodegenerative disorders》**
*Authors: Chen L., Park J., Smith E.*
摘要：利用杆状病毒-昆虫细胞系统表达重组CWF19L2蛋白，结合质谱技术鉴定其与神经退行性疾病相关蛋白TDP-43的互作，提示其在神经元DNA损伤修复中的作用。
**备注**：建议通过PubMed、Google Scholar或Web of Science以关键词“CWF19L2 recombinant protein”或“CWF19L2 functional study”检索真实文献。实际研究中需结合具体方向筛选(如表达系统、疾病模型等)。
**Background of Recombinant Human CWF19L2 Protein**
The CWF19L2 (Crn/Twig family member 19-like 2) protein is a conserved eukaryotic protein implicated in cell cycle regulation and RNA splicing. It shares homology with yeast Cwf19. a component of the NineTeen Complex (NTC), which plays roles in spliceosome assembly and pre-mRNA processing. In humans, CWF19L2 is hypothesized to interact with spliceosomal machinery, influencing mRNA maturation, and may contribute to mitotic progression by regulating spindle assembly checkpoint signaling.
Structurally, CWF19L2 contains predicted WD40 repeat domains, suggesting involvement in protein-protein interactions. Dysregulation of CWF19L2 has been linked to genomic instability and diseases, including cancer, where altered splicing or cell cycle errors drive tumorigenesis.
Recombinant human CWF19L2 protein is produced via expression systems (e.g., *E. coli* or mammalian cells*) for functional studies. Its purified form enables exploration of molecular mechanisms in splicing, cell division, and disease pathways. Research using this protein aims to clarify its role in maintaining genome integrity and potential as a therapeutic target. Current studies also investigate its interactions with spliceosome components or cell cycle regulators, bridging gaps in understanding RNA processing and mitotic fidelity.
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