| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CUL4A |
| Uniprot No | Q13619 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-759aa |
| 氨基酸序列 | MADEAPRKGSFSALVGRTNGLTKPAALAAAPAKPGGAGGSKKLVIKNFRDRPRLPDNYTQDTWRKLHEAVRAVQSSTSIRYNLEELYQAVENLCSHKVSPMLYKQLRQACEDHVQAQILPFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTYVLQNSTLPSIWDMGLELFRTHIISDKMVQSKTIDGILLLIERERSGEAVDRSLLRSLLGMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQEREVPEYLNHVSKRLEEEGDRVITYLDHSTQKPLIACVEKQLLGEHLTAILQKGLDHLLDENRVPDLAQMYQLFSRVRGGQQALLQHWSEYIKTFGTAIVINPEKDKDMVQDLLDFKDKVDHVIEVCFQKNERFVNLMKESFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERTLDKIMILFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQSDSGPIDLTVNILTMGYWPTYTPMEVHLTPEMIKLQEVFKAFYLGKHSGRKLQWQTTLGHAVLKAEFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNGEFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDNPNQYHYVA |
| 分子量 | 114.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CUL4A蛋白的3篇示例参考文献(内容基于研究领域常见主题，但需根据实际文献调整)：
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1. **文献名称**: *"Functional characterization of recombinant human CUL4A in the CRL4 ubiquitin ligase complex"*
**作者**: Jackson, S. et al.
**摘要**: 研究利用大肠杆菌表达系统重组表达了人CUL4A蛋白，验证其与DDB1的相互作用，并证明其在体外泛素化反应中调控底物降解的功能，揭示了CUL4A在DNA损伤应答中的分子机制。
2. **文献名称**: *"Structural insights into the assembly of the CUL4A-DDB1 E3 ubiquitin ligase complex"*
**作者**: Chen, Z. & Guo, Z.
**摘要**: 通过重组CUL4A蛋白的结晶学分析，解析了其与DDB1结合的结构域，阐明了CRL4复合体组装的关键界面，为靶向该复合体的药物设计提供依据。
3. **文献名称**: *"Recombinant CUL4A expression and its role in cell cycle regulation"*
**作者**: Harper, J.W. et al.
**摘要**: 利用哺乳动物细胞系统表达重组CUL4A，发现其通过泛素化降解周期蛋白依赖性激酶抑制剂p27.直接调控G1/S期转换，暗示其在癌症中的潜在作用。
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**注**：以上文献为示例，实际引用时建议通过PubMed或Web of Science等平台检索真实文献(例如搜索关键词：CUL4A recombinant, CRL4 ubiquitin ligase)。若需具体论文，请提供更详细的研究方向。
Cullin 4A (CUL4A) is a member of the cullin protein family, which serves as a scaffold for the assembly of Cullin-RING E3 ubiquitin ligase complexes. These complexes play critical roles in ubiquitination, a post-translational modification that directs substrates for proteasomal degradation or functional modulation. CUL4A interacts with DNA damage-binding protein 1 (DDB1) and other substrate receptors (e.g., CRBN, DCAFs) to form the CRL4A complex, targeting proteins involved in DNA repair, cell cycle regulation, chromatin remodeling, and stress responses. Dysregulation of CUL4A is linked to cancers, neurological disorders, and viral pathogenesis due to its role in controlling genome stability and signaling pathways.
Recombinant human CUL4A protein, produced via expression systems like *E. coli* or mammalian cells, retains structural and functional integrity for *in vitro* studies. It enables researchers to explore substrate recruitment, ubiquitination mechanisms, and interactions with regulatory proteins (e.g., COP9 signalosome). Applications include drug screening, biochemical assays (e.g., ubiquitination kinetics), and structural studies. Purified CUL4A often includes affinity tags (e.g., GST, His-tag) to facilitate isolation. Its production supports investigations into disease mechanisms and therapeutic targeting of the ubiquitin-proteasome system.
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