| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTK6 |
| Uniprot No | Q13882 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-451aa |
| 氨基酸序列 | VSRDQAHLGPKYVGLWDFKSRTDEELSFRAGDVFHVARKEEQWWWATLLD EAGGAVAQGYVPHNYLAERETVESEPWFFGCISRSEAVRRLQAEGNATGA FLIRVSEKPSADYVLSVRDTQAVRHYKIWRRAGGRLHLNEAVSFLSLPEL VNYHRAQSLSHGLRLAAPCRKHEPEPLPHWDDWERPREEFTLCRKLGSGY FGEVFEGLWKDRVQVAIKVISRDNLLHQQMLQSEIQAMKKLRHKHILALY AVVSVGDPVYIITELMAKGSLLELLRDSDEKVLPVSELLDIAWQVAEGMC YLESQNYIHRDLAARNILVGENTLCKVGDFGLARLIKEDVYLSHDHNIPY KWTAPEALSRGHYSTKSDVWSFGILLHEMFSRGQVPYPGMSNHEAFLRVD AGYRMPCPLECPPSVHKLMLTCWCRDPEQRPCFKALRERLSSFTSYENPT |
| 预测分子量 | 80 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTK6重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Expression, purification, and characterization of active tyrosine kinase Brk (PTK6) from Escherichia coli"*
**作者**：Chen H, et al.
**摘要**：研究报道了在大肠杆菌中成功表达并纯化重组PTK6蛋白，通过His标签亲和层析获得高纯度蛋白，并验证其酪氨酸激酶活性，为后续功能研究提供基础。
2. **文献名称**：*"Structural insights into the functional role of PTK6 in HER2-positive breast cancer via recombinant protein analysis"*
**作者**：Patel R, et al.
**摘要**：通过重组PTK6蛋白的体外实验，揭示了其在HER2阳性乳腺癌细胞中的信号调控机制，证明PTK6与HER2相互作用并促进肿瘤细胞迁移，为靶向治疗提供依据。
3. **文献名称**：*"Kinase domain truncation of recombinant PTK6 alters substrate specificity and cellular localization"*
**作者**：Lee S, et al.
**摘要**：研究构建了PTK6激酶结构域截短的重组蛋白，发现其底物选择性与全长的差异，并观察到亚细胞定位改变，提示激酶结构域对PTK6功能的关键调控作用。
4. **文献名称**：*"Development of a mammalian cell expression system for producing phosphorylated PTK6 for drug screening"*
**作者**：Zhang Y, et al.
**摘要**：利用哺乳动物细胞系统表达重组PTK6蛋白，获得具有生理磷酸化修饰的活性形式，成功应用于激酶抑制剂的高通量筛选，推动抗癌药物开发。
(注：以上文献信息为模拟概括，实际引用需根据具体论文内容调整。)
**Background of PTK6 Recombinant Protein**
PTK6 (Protein tyrosine kinase 6), also known as Brk (Breast tumor kinase), is an intracellular non-receptor tyrosine kinase belonging to the Src kinase family. Unlike classical Src kinases, PTK6 lacks a regulatory phosphorylation site and a myristoylation signal, resulting in distinct subcellular localization and activation mechanisms. It is primarily expressed in epithelial tissues, including the skin, gastrointestinal tract, and mammary glands, where it regulates signaling pathways involved in cell proliferation, differentiation, migration, and apoptosis. PTK6 contains SH3 and SH2 domains that mediate protein-protein interactions, enabling its role in signal transduction by phosphorylating downstream targets like STAT3. IRS-4. and β-catenin.
The dual role of PTK6 in cancer has garnered significant interest. While it exhibits tumor-promoting activity in certain cancers (e.g., breast, colon) by enhancing oncogenic signaling (e.g., EGFR, HER2), it acts as a tumor suppressor in others (e.g., prostate, skin) by inhibiting pro-survival pathways. This context-dependent behavior underscores its complex regulatory network and tissue-specific functions.
Recombinant PTK6 protein, produced via expression systems such as *E. coli* or mammalian cells, retains enzymatic activity and is widely used to study its biochemical properties, substrate interactions, and therapeutic potential. Purification tags (e.g., His-tag) facilitate isolation, enabling applications in kinase assays, drug screening, and structural studies. Researchers also employ PTK6 recombinant protein to explore its role in pathological conditions, including metastasis and drug resistance, and to develop targeted inhibitors for cancer therapy.
Overall, PTK6 represents a promising yet challenging target due to its multifaceted roles, driving ongoing research to elucidate its mechanisms and therapeutic relevance.
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