| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTNNAL1 |
| Uniprot No | Q9UBT7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-734aa |
| 氨基酸序列 | MAASPGPAGV GGAGAVYGSG SSGFALDSGL EIKTRSVEQT LLPLVSQITT LINHKDNTKK SDKTLQAIQR VGQAVNLAVG RFVKVGEAIA NENWDLKEEI NIACIEAKQA GETIAALTDI TNLNHLESDG QITIFTDKTG VIKAARLLLS SVTKVLLLAD RVVIKQIITS RNKVLATMER LEKVNSFQEF VQIFSQFGNE MVEFAHLSGD RQNDLKDEKK KAKMAAARAV LEKCTMMLLT ASKTCLRHPN CESAHKNKEG VFDRMKVALD KVIEIVTDCK PNGETDISSI SIFTGIKEFK MNIEALRENL YFQSKENLSV TLEVILERME DFTDSAYTSH EHRERILELS TQARMELQQL ISVWIQAQSK KTKSIAEELE LSILKISHSL NELKKELHST ATQLAADLLK YHADHVVLKA LKLTGVEGNL EALAEYACKL SEQKEQLVET CRLLRHISGT EPLEITCIHA EETFQVTGQQ IISAAETLTL HPSSKIAKEN LDVFCEAWES QISDMSTLLR EINDVFEGRR GEKYGYLSLP KPMKNNANLK SLKPDKPDSE EQAKIAKLGL KLGLLTSDAD CEIEKWEDQE NEIVQYGRNM SSMAYSLYLF TRGEGPLKTS QDLIHQLEVF AAEGLKLTSS VQAFSKQLKD DDKLMLLLEI NKLIPLCHQL QTVTKTSLQN KVFLKVDKCI TKTRSMMALL VQLLSLCYKL LKKLQMENNG WVSVTNKDTM DSKT |
| 分子量 | 81.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CTNNAL1蛋白的虚构参考文献示例(仅供参考)：
1. **文献名称**：*Expression and Functional Characterization of Recombinant Human CTNNAL1 in Epithelial Cell Adhesion*
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌系统中表达并纯化了重组人CTNNAL1蛋白，证实其通过调节E-钙黏蛋白复合物增强上皮细胞间黏附，并揭示其与β-catenin的互作机制。
2. **文献名称**：*CTNNAL1 Modulates Wnt/β-Catenin Signaling via Recombinant Protein Interaction Assays*
**作者**：Zhang L, et al.
**摘要**：通过体外重组CTNNAL1蛋白实验，发现其竞争性结合β-catenin抑制Wnt信号通路，为结直肠癌中CTNNAL1的抑癌作用提供分子证据。
3. **文献名称**：*Role of Recombinant CTNNAL1 in TGF-β-Induced Fibrosis: Insights from Structural Analysis*
**作者**：Jones R, et al.
**摘要**：利用重组CTNNAL1蛋白解析其晶体结构，揭示其通过结合Smad3调控TGF-β介导的纤维化进程，提示其作为肺纤维化治疗的潜在靶点。
4. **文献名称**：*Development of a CTNNAL1 Knockout Model Using Recombinant Protein-Based Screening*
**作者**：Chen X, et al.
**摘要**：基于重组CTNNAL1的功能验证建立基因编辑细胞模型，证明其缺失导致细胞迁移能力异常，提示其在胚胎发育中的关键作用。
注：上述内容为示例性虚构，实际研究需参考真实文献数据库(如PubMed)。
Recombinant human CTNNAL1 (Catenin Alpha-Like 1) protein is a key regulatory molecule involved in cell adhesion and signaling pathways. As a member of the α-catenin family, CTNNAL1 interacts with β-catenin and actin cytoskeleton components, playing a dual role in stabilizing adherens junctions and modulating Wnt/β-catenin signaling. It regulates cell-cell adhesion by linking the E-cadherin/β-catenin complex to the actin network, critical for maintaining epithelial integrity and barrier function. Additionally, CTNNAL1 influences cell motility, proliferation, and differentiation, with dysregulation implicated in cancer metastasis, fibrosis, and inflammatory disorders.
Structurally, CTNNAL1 contains conserved N- and C-terminal domains that mediate interactions with β-catenin and cytoskeletal proteins. Recombinant CTNNAL1 is typically expressed in bacterial or mammalian systems with purification tags (e.g., GST or His-tag) to facilitate experimental applications. It serves as a tool to study molecular mechanisms of cell adhesion, Wnt signaling crosstalk, and epithelial-mesenchymal transition (EMT) in disease models. Researchers utilize this protein in binding assays, structural analysis, and inhibitor screening to explore therapeutic strategies targeting CTNNAL1-associated pathways in oncology and chronic inflammatory diseases.
×
