| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTDSPL2 |
| Uniprot No | Q05D32 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-466aa |
| 氨基酸序列 | MRLRTRKASQQSNQIQTQRTARAKRKYSEVDDSLPSGGEKPSKNETGLLSSIKKFIKGSTPKEERENPSKRSRIERDIDNNLITSTPRAGEKPNKQISRVRRKSQVNGEAGSYEMTNQHVKQNGKLEDNPSSGSPPRTTLLGTIFSPVFNFFSPANKNGTSGSDSPGQAVEAEEIVKQLDMEQVDEITTSTTTSTNGAAYSNQAVQVRPSLNNGLEEAEETVNRDIPPLTAPVTPDSGYSSAHAEATYEEDWEVFDPYYFIKHVPPLTEEQLNRKPALPLKTRSTPEFSLVLDLDETLVHCSLNELEDAALTFPVLFQDVIYQVYVRLRPFFREFLERMSQMYEIILFTASKKVYADKLLNILDPKKQLVRHRLFREHCVCVQGNYIKDLNILGRDLSKTIIIDNSPQAFAYQLSNGIPIESWFMDKNDNELLKLIPFLEKLVELNEDVRPHIRDRFRLHDLLPPD |
| 分子量 | 79.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CTDSPL2蛋白的模拟参考文献示例(注：文献为虚构，仅供参考格式，实际需通过学术数据库检索验证)：
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1. **文献名称**: *"Expression and functional characterization of recombinant human CTDSPL2 phosphatase"*
**作者**: Zhang Y, Li H, Wang J.
**摘要**: 该研究成功在大肠杆菌中表达并纯化了重组人CTDSPL2蛋白，验证其具有CTD尾部的丝氨酸磷酸酶活性，并发现其通过去磷酸化RNA聚合酶II调控转录终止过程。
2. **文献名称**: *"CTDSPL2 modulates TGF-β signaling via SMAD dephosphorylation"*
**作者**: Kumar S, Patel R, Lee TH.
**摘要**: 研究利用重组CTDSPL2蛋白进行体外实验，证实其通过去磷酸化SMAD蛋白抑制TGF-β信号通路，提示其在细胞分化与肿瘤抑制中的潜在作用。
3. **文献名称**: *"Structural insights into CTDSPL2 catalytic mechanism by X-ray crystallography"*
**作者**: Chen X, Ito T, Nakayama K.
**摘要**: 通过X射线晶体学解析了重组人CTDSPL2蛋白的三维结构，揭示了其活性位点关键氨基酸残基，为开发靶向该酶的抑制剂奠定基础。
4. **文献名称**: *"CTDSPL2 deficiency promotes neuronal apoptosis via AKT pathway dysregulation"*
**作者**: Wang L, Zhang Q, Xu P.
**摘要**: 利用重组CTDSPL2蛋白补偿实验，证明其在神经元中通过调控AKT磷酸化水平抑制凋亡，提示其与神经退行性疾病的相关性。
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**建议**：实际研究中，请通过 **PubMed**、**Google Scholar** 或 **Web of Science** 检索最新文献，结合关键词如“CTDSPL2 recombinant”“CTDSPL2 phosphatase function”获取真实数据。该蛋白研究可能涉及转录调控、信号通路(如TGF-β)或疾病机制等领域。
**Background of Recombinant Human CTDSPL2 Protein**
CTDSPL2 (CTD Small Phosphatase-Like 2), also known as HSPC129 or SCP4. is a member of the small C-terminal domain phosphatase (SCP) family, which plays a role in regulating transcription and cellular signaling. This protein contains a conserved phosphatase domain and shares structural homology with other CTD phosphatases, such as CTDSP1 and CTDSPL. CTDSPL2 is implicated in dephosphorylating the C-terminal domain (CTD) of RNA polymerase II, a critical process for transcription elongation, termination, and coupling with mRNA processing.
Functionally, CTDSPL2 is linked to the TGF-β/BMP and Wnt signaling pathways, potentially modulating Smad or β-catenin activity, though its exact mechanisms remain under investigation. Studies suggest its involvement in cell cycle regulation, differentiation, and tumor suppression, with altered expression observed in cancers, including gliomas and hepatocellular carcinoma.
Recombinant human CTDSPL2 protein is engineered using mammalian or bacterial expression systems to enable in vitro studies. It serves as a tool for elucidating enzymatic activity, substrate interactions, and roles in transcriptional or signaling networks. Research applications include enzyme kinetics, protein-protein interaction assays, and exploring its therapeutic potential in diseases linked to CTD phosphorylation dysregulation.
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