| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Ctf2 |
| Uniprot No | P83714 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-204aa |
| 氨基酸序列 | APISPSEP IGQAYSLALY MQKNTSALLQ TYLQHQGSPF SDPGFSAPEL QLSTLPSAAV SFKTWHAMED AERLSRAQGA FLALTQHLQL VGDDQSYLNP GSPILLAQLG AARLRAQGLL GNMAAIMTAL GLPIPPEEDT LGFVPFGASA FERKCRGYIV TREYGHWTDR AVRDLALLKA KYSA |
| 分子量 | 22 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设构造的关于重组小鼠Ctf2蛋白的研究文献示例(请注意，这些文献为虚拟示例，实际引用需核实)：
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1. **文献名称**: *"Recombinant Expression and Functional Characterization of Mouse Ctf2 in Sister Chromatid Cohesion"*
**作者**: Tanaka et al.
**摘要**: 本研究通过在大肠杆菌中表达并纯化重组小鼠Ctf2蛋白，探究其在姐妹染色单体内聚中的作用。实验证实Ctf2与cohesin复合体结合，并揭示了其ATP酶活性对染色体稳定的重要性。
2. **文献名称**: *"Structural Insights into Mouse Ctf2 Using Cryo-EM: A Recombinant Protein Approach"*
**作者**: Zhang et al.
**摘要**: 作者利用昆虫细胞系统表达重组小鼠Ctf2.通过冷冻电镜解析其三维结构，发现其N端结构域对染色体分离的关键作用，为理解有丝分裂机制提供新依据。
3. **文献名称**: *"Development of a Ctf2 Knockout Mouse Model and Rescue via Recombinant Protein Delivery"*
**作者**: Gupta et al.
**摘要**: 研究通过重组Ctf2蛋白回补实验，验证其在胚胎发育中的必要性。纯化的蛋白成功恢复了敲除小鼠模型的染色体分离缺陷，证明Ctf2对早期胚胎存活至关重要。
4. **文献名称**: *"Optimization of Mouse Ctf2 Protein Production in a Mammalian Expression System"*
**作者**: Lee & Kim
**摘要**: 本研究比较了不同表达系统(哺乳动物细胞、大肠杆菌)对重组Ctf2蛋白活性的影响，发现哺乳动物系统更有利于其正确折叠及功能，为后续互作研究奠定基础。
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**备注**：若需真实文献，建议使用关键词(如“recombinant mouse Ctf2”“chromosome cohesion”)在PubMed或Google Scholar检索，并核实基因名称准确性(例如，确认Ctf2是否为同源物或别名)。
Recombinant murine Ctf2 (Chromosome transmission fidelity protein 2) is a conserved protein involved in maintaining genomic stability during chromosome segregation and sister chromatid cohesion. In mammals, Ctf2 (also known as Eco1/Ctf7 in yeast homologs) functions as an acetyltransferase essential for establishing cohesion between replicated sister chromatids by acetylating cohesin complex subunits. This post-translational modification stabilizes cohesin loading onto DNA during S phase, ensuring accurate chromosome segregation in mitosis. Studies in mice highlight its critical role in embryonic development, cell cycle progression, and preventing aneuploidy. Recombinant mouse Ctf2 protein is typically produced in bacterial or eukaryotic expression systems to investigate its enzymatic activity, structure-function relationships, and interactions with cohesin components (e.g., Smc3). Its dysfunction is linked to chromosomal instability phenotypes relevant to cancer and developmental disorders. Research using purified recombinant Ctf2 has advanced understanding of replication-coupled cohesion establishment and provided tools for screening potential modulators of cohesion processes. The protein’s conserved N-terminal acetyltransferase domain and C-terminal regulatory regions remain focal points for mechanistic studies.
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