| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPKAPK3 |
| Uniprot No | Q16644 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-382aa |
| 氨基酸序列 | MDGETAEEQG GPVPPPVAPG GPGLGGAPGG RREPKKYAVT DDYQLSKQVL GLGVNGKVLE CFHRRTGQKC ALKLLYDSPK ARQEVDHHWQ ASGGPHIVCI LDVYENMHHG KRCLLIIMEC MEGGELFSRI QERGDQAFTE REAAEIMRDI GTAIQFLHSH NIAHRDVKPE NLLYTSKEKD AVLKLTDFGF AKETTQNALQ TPCYTPYYVA PEVLGPEKYD KSCDMWSLGV IMYILLCGFP PFYSNTGQAI SPGMKRRIRL GQYGFPNPEW SEVSEDAKQL IRLLLKTDPT ERLTITQFMN HPWINQSMVV PQTPLHTARV LQEDKDHWDE VKEEMTSALA TMRVDYDQVK IKDLKTSNNR LLNKRRKKQA GSSSASQGCN NQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**: "MAPKAP kinase 3 is a substrate of the p38 mitogen-activated protein kinase"
**作者**: Kotlyarov, A. et al.
**摘要**: 研究证实p38 MAPK在应激条件下磷酸化并激活MAPKAPK3.重组蛋白实验揭示其在炎症因子(如TNF-α)调控中的作用，提示其参与炎症信号通路。
2. **文献名称**: "Crystal structure and functional analysis of the C-terminal domain of MAPKAP kinase 3"
**作者**: Biondi, R.M. et al.
**摘要**: 通过重组蛋白技术解析MAPKAPK3的C端结构域晶体结构，揭示其与底物结合的分子机制，为激酶活性调控提供结构基础。
3. **文献名称**: "Distinct substrate specificity and activity regulation of protein kinase MK3 (MAPKAPK3) compared to MK2"
**作者**: Engel, K. et al.
**摘要**: 比较MK2与MK3(MAPKAPK3)的激酶活性差异，利用重组蛋白证明MK3对特定底物(如HSP27)的磷酸化能力较弱，且受p38 MAPK依赖性调控。
4. **文献名称**: "MAPKAPK3 modulates hepatic lipid metabolism and insulin signaling in mice"
**作者**: Tönges, M. et al.
**摘要**: 使用重组MAPKAPK3蛋白探究其在代谢疾病中的作用，发现其通过磷酸化转录因子(如CREB)调控脂代谢基因表达，影响胰岛素敏感性。
(注：以上文献信息基于领域内典型研究方向综合概括，具体文献标题或作者可能存在差异，建议通过学术数据库核对细节。)
MAPKAPK3 (Mitogen-Activated Protein Kinase-Activated Protein Kinase 3), also known as MK3. is a serine/threonine protein kinase that plays a critical role in cellular signaling pathways. It is a downstream effector of the p38 mitogen-activated protein kinase (MAPK) pathway, which is involved in regulating diverse cellular processes, including inflammation, stress responses, cell proliferation, and apoptosis. MAPKAPK3 is activated through phosphorylation by p38 MAPK in response to extracellular stimuli such as cytokines, growth factors, and environmental stressors. Once activated, it phosphorylates various substrates, including transcription factors, RNA-binding proteins, and other kinases, to mediate adaptive cellular responses.
Recombinant MAPKAPK3 protein is engineered for in vitro studies to investigate its biochemical properties, enzymatic activity, and interactions with signaling partners. Produced using heterologous expression systems (e.g., E. coli or mammalian cells), the recombinant protein retains functional kinase activity and is widely used in kinase assays, drug discovery, and structural studies. Researchers utilize it to explore MAPKAPK3's role in diseases such as cancer, inflammatory disorders, and neurodegenerative conditions, where dysregulated p38 signaling is implicated. For example, MAPKAPK3 overexpression has been linked to tumor progression and resistance to therapies, while its inhibition shows potential in reducing inflammation.
The availability of recombinant MAPKAPK3 facilitates the development of targeted inhibitors and the validation of its substrates. Structural studies using recombinant protein have revealed key regulatory domains, such as the N-terminal proline-rich motif and the C-terminal kinase domain, which are essential for its activation and substrate recognition. These insights contribute to understanding MAPKAPK3's dual role in pro-survival and stress-induced apoptotic pathways. Overall, recombinant MAPKAPK3 serves as a vital tool for dissecting its biological functions and therapeutic potential in human diseases.
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