| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DYRK3 |
| Uniprot No | O43781-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 63-568aa |
| 氨基酸序列 | MKVEQLFQEFGNRKSNTIQSDGISDSEKCSPTVSQGKSSDCLNTVKSNSS SKAPKVVPLTPEQALKQYKHHLTAYEKLEIINYPEIYFVGPNAKKRHGVI GGPNNGGYDDADGAYIHVPRDHLAYRYEVLKIIGKGSFGQVARVYDHKLR QYVALKMVRNEKRFHRQAAEEIRILEHLKKQDKTGSMNVIHMLESFTFRN HVCMAFELLSIDLYELIKKNKFQGFSVQLVRKFAQSILQSLDALHKNKII HCDLKPENILLKHHGRSSTKVIDFGSSCFEYQKLYTYIQSRFYRAPEIIL GSRYSTPIDIWSFGCILAELLTGQPLFPGEDEGDQLACMMELLGMPPPKL LEQSKRAKYFINSKGIPRYCSVTTQADGRVVLVGGRSRRGKKRGPPGSKD WGTALKGCDDYLFIEFLKRCLHWDPSARLTPAQALRHPWISKSVPRPLTT IDKVSGKRVVNPASAFQGLGSKLPPVVGIANKLKANLMSETNGSIPLCSV LPKLIS |
| 预测分子量 | 95 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DYRK3重组蛋白的3篇模拟参考文献及摘要概括：
1. **文献名称**：*DYRK3 regulates stress granule dynamics and cellular adaptation to glucose deprivation*
**作者**：H. Xie et al.
**摘要**：研究揭示了DYRK3重组蛋白通过磷酸化关键应激颗粒蛋白(如G3BP1)调控应激颗粒的组装与解聚，影响细胞在葡萄糖缺乏条件下的存活与代谢适应。
2. **文献名称**：*Structural and functional analysis of DYRK3 kinase using recombinant expression systems*
**作者**：M. Tanaka, R. Kumar
**摘要**：通过大肠杆菌重组表达系统获得高纯度DYRK3蛋白，结合晶体结构解析和体外激酶实验，明确了其ATP结合域的特异性及底物识别机制。
3. **文献名称**：*DYRK3-mediated eIF2B phosphorylation promotes translational recovery post-stress*
**作者**：J. Chen et al.
**摘要**：该研究利用重组DYRK3蛋白验证其与eIF2B的相互作用，证明DYRK3通过磷酸化eIF2B调控蛋白质翻译重启，对细胞应对内质网应激至关重要。
(注：以上文献为模拟概括，实际研究需参考真实发表的学术论文。)
**Background of DYRK3 Recombinant Protein**
DYRK3 (Dual-specificity tyrosine-regulated kinase 3) belongs to the DYRK family of protein kinases, which are evolutionarily conserved regulators of cellular processes. The DYRK family includes five members (DYRK1A, DYRK1B, DYRK2. DYRK3. and DYRK4) classified under the CMGC group of kinases, characterized by their dual-specificity phosphorylation activity on serine/threonine and tyrosine residues. DYRK3 is distinct in its tissue distribution and functional roles, with expression observed in various organs, including the brain, heart, and testes.
Functionally, DYRK3 is implicated in stress response pathways, particularly through its regulation of biomolecular condensates like stress granules and the mTORC1 signaling axis. It modulates cellular adaptation to environmental stressors (e.g., nutrient deprivation, oxidative stress) by influencing protein phase separation and autophagy. Studies also link DYRK3 to cancer progression, neurodegeneration, and viral infection responses, highlighting its role in cell survival and apoptosis.
Recombinant DYRK3 protein is engineered for in vitro studies to dissect its kinase activity, substrate interactions, and structural properties. Produced using expression systems like *E. coli* or mammalian cells, it is purified to homogeneity for biochemical assays, inhibitor screening, and structural biology (e.g., crystallography). Researchers utilize this tool to explore DYRK3's regulatory mechanisms, including autophosphorylation and substrate specificity, which are critical for developing targeted therapies.
Interest in DYRK3 has grown due to its potential as a therapeutic target, particularly in diseases involving dysregulated stress responses or protein aggregation. Inhibitors of DYRK3 are being investigated for their ability to modulate pathological processes, such as tumor growth or neurodegenerative proteinopathies. Overall, DYRK3 recombinant protein serves as a vital resource for unraveling its biological significance and translational applications.
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