| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSTF2T |
| Uniprot No | Q9H0L4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-616aa |
| 氨基酸序列 | MSSLAVRDPAMDRSLRSVFVGNIPYEATEEQLKDIFSEVGSVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRVDNAASEKNKEELKSLGPAAPIIDSPYGDPIDPEDAPESITRAVASLPPEQMFELMKQMKLCVQNSHQEARNMLLQNPQLAYALLQAQVVMRIMDPEIALKILHRKIHVTPLIPGKSQSVSVSGPGPGPGPGLCPGPNVLLNQQNPPAPQPQHLARRPVKDIPPLMQTPIQGGIPAPGPIPAAVPGAGPGSLTPGGAMQPQLGMPGVGPVPLERGQVQMSDPRAPIPRGPVTPGGLPPRGLLGDAPNDPRGGTLLSVTGEVEPRGYLGPPHQGPPMHHASGHDTRGPSSHEMRGGPLGDPRLLIGEPRGPMIDQRGLPMDGRGGRDSRAMETRAMETEVLETRVMERRGMETCAMETRGMEARGMDARGLEMRGPVPSSRGPMTGGIQGPGPINIGAGGPPQGPRQVPGISGVGNPGAGMQGTGIQGTGMQGAGIQGGGMQGAGIQGVSIQGGGIQGGGIQGASKQGGSQPSSFSPGQSQVTPQDQEKAALIMQVLQLTADQIAMLPPEQRQSILILKEQIQKSTGAS |
| 分子量 | 90.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于重组人CSTF2T蛋白的参考文献示例，供参考(建议通过学术数据库验证实际文献)：
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1. **文献名称**: *Expression and Functional Analysis of Recombinant Human CSTF2T in mRNA Polyadenylation*
**作者**: Thompson, R., et al. (2015)
**摘要**: 本研究在大肠杆菌中表达了重组人CSTF2T蛋白，验证其与CstF复合体的相互作用，证明其在体外poly(A)信号选择中的调控作用。
2. **文献名称**: *CSTF2T Structural Characterization and RNA-Binding Specificity*
**作者**: Li, S., & Manley, J. L. (2008)
**摘要**: 通过X射线晶体学解析CSTF2T的RNA结合结构域结构，揭示其与富含GU序列的RNA结合机制，为理解3'端加工提供结构基础。
3. **文献名称**: *CSTF2T Splicing Variants in Male Germ Cells: Implications for Infertility*
**作者**: Zhang, Y., et al. (2020)
**摘要**: 发现CSTF2T在睾丸特异性表达，敲除小鼠模型显示精子发生异常，提示其与男性不育的潜在关联。
4. **文献名称**: *Tissue-Specific Polyadenylation Regulated by CSTF2T Isoforms*
**作者**: Dass, B., & MacDonald, C. C. (2003)
**摘要**: 比较CSTF2不同亚型(包括CSTF2T)在生殖细胞中的活性，阐明其通过选择性poly(A)信号调控基因表达的作用。
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**注**：以上文献为示例性内容，实际研究请通过PubMed、Google Scholar等平台以关键词“CSTF2T”、“CstF-64”、“recombinant CSTF2T”查询最新或经典文献。
**Background of Recombinant Human CSTF2T Protein**
The Cleavage Stimulation Factor (CsfF) complex is essential for mRNA 3' end processing, a critical step in eukaryotic gene expression. CSTF2T (Cleavage Stimulation Factor Subunit 2 Tau variant) is a subunit of the CsfF complex, which recognizes polyadenylation signals and facilitates pre-mRNA cleavage and poly-A tail addition. Unlike its paralog CSTF2. CSTF2T is primarily expressed in the testis and germ cells, suggesting specialized roles in reproduction and germline development.
Recombinant human CSTF2T protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its molecular interactions, structural features, and regulatory mechanisms. It retains RNA-binding activity, enabling functional analyses of its role in poly-A site selection and mRNA processing. Studies using recombinant CSTF2T have highlighted its tissue-specific interactions with other CsfF subunits (e.g., CSTF1. CSTF3) and its potential involvement in male infertility when dysregulated.
Additionally, CSTF2T’s unique expression pattern and conserved domains make it a focal point for investigating mRNA processing variations across tissues or diseases, such as cancer or developmental disorders. Recombinant protein tools aid in deciphering its contributions to transcript diversity and post-transcriptional regulation.
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