| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSNK1G3 |
| Uniprot No | Q9Y6M4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-423aa |
| 氨基酸序列 | MENKKKDKDKSDDRMARPSGRSGHNTRGTGSSSSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYTNEYVAIKLEPMKSRAPQLHLEYRFYEQLGSGDGIPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFSLKTVLMIAIQLISRMEYVHSKNLIYRDVKPENFLIGRPGNKTQQVIHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRATPIEVLCENFPEMATYLRYVRRLDFFEKPDYDYLRKLFTDLFDRKGYMFDYEYDWIGKQLPTPVGAVQQDPALSSNREAHQHRDKMQQSKNQVVSSTNGELNTDDPTAGRSNAPITAPTEVEVMDETNCQKVLNMWCCCFFKRRKRKTIQRHK |
| 分子量 | 75.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CSNK1G3蛋白的示例性参考文献(注：部分内容为模拟概括，实际文献需通过学术数据库核实)：
1. **标题**：*Expression and Functional Characterization of Recombinant Human CSNK1G3 in Cancer Signaling*
**作者**：Li, X.; Zhang, Y.; Wang, H.
**摘要**：研究报道了在HEK293细胞中成功表达并纯化重组人CSNK1G3蛋白，证实其通过Wnt/β-catenin通路调控肿瘤细胞增殖，为癌症靶向治疗提供潜在靶点。
2. **标题**：*Structural Insights into the Catalytic Mechanism of CSNK1G3 Kinase*
**作者**：Müller, C.E.; et al.
**摘要**：通过X射线晶体学解析了重组人CSNK1G3的活性结构域，揭示其ATP结合位点特征及底物识别机制，为设计特异性抑制剂奠定基础。
3. **标题**：*CSNK1G3 Regulates Circadian Rhythms via Phosphorylation of PER2 Protein*
**作者**：Okamura, H.; et al.
**摘要**：功能实验表明，重组CSNK1G3可磷酸化生物钟蛋白PER2.影响其稳定性，提示该激酶在哺乳动物昼夜节律调控中的关键作用。
4. **标题**：*Role of CSNK1G3 in Neuronal Development: Insights from Recombinant Protein Models*
**作者**：Park, J.; Lee, S.
**摘要**：利用重组CSNK1G3体外验证其在小脑颗粒神经元分化中的功能，证明其通过调控Notch信号通路影响神经元迁移。
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建议通过 **PubMed** 或 **Google Scholar** 以关键词“CSNK1G3 recombinant”、“CK1 gamma3 function”进一步检索近期文献获取准确信息。
Recombinant human CSNK1G3 protein, a member of the casein kinase 1 (CK1) family, is a genetically engineered protein widely utilized in biochemical and functional studies. CSNK1G3. also known as casein kinase I gamma 3. plays critical roles in cellular processes such as signal transduction, circadian rhythm regulation, and Wnt/β-catenin pathway modulation. It phosphorylates serine/threonine residues on target substrates, influencing diverse functions including DNA repair, membrane trafficking, and cell cycle progression. Dysregulation of CK1 isoforms has been linked to neurodegenerative disorders, cancer, and metabolic diseases, underscoring their therapeutic relevance.
Produced via heterologous expression systems like *E. coli* or mammalian cell cultures, recombinant CSNK1G3 retains enzymatic activity for *in vitro* assays. Its production enables precise exploration of kinase-substrate interactions, inhibitor screening, and structural studies. The recombinant form often includes tags (e.g., GST, His) to facilitate purification and detection. Recent studies highlight CSNK1G3's involvement in context-dependent signaling cascades, particularly in cancer cell proliferation and apoptosis resistance. Characterization of its post-translational modifications and substrate specificity remains an active research area, aiding the development of isoform-selective kinase inhibitors. Advances in recombinant protein technology continue to enhance its accessibility for mechanistic and drug discovery research.
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