| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAMK1D |
| Uniprot No | Q8IU85 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-385aa |
| 氨基酸序列 | MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLVPRGSMARENGESSSSWKKQAEDIKKIFE FKETLGTGAFSEVVLAEEKATGKLFAVKCIPKKALKGKESSIENEIAVLR KIKHENIVALEDIYESPNHLYLVMQ LVSGGELFDRIVEKGFYTEKDASTLIRQVLDAVYYLHRMGIVHRDLKPEN LLYYSQDEESKIMISDFGLSKMEGKGDVMSTACGTPGYVAPEVLAQKPYS KAVDCWSIGVIAYILLCGYPPFYDENDSKLFEQILKAEYEFDSPYWDDIS DSAKDFIRNLMEKDPNKRYTCEQAARHPWIAGDTALNKNIHESVSAQIRK NFAKSKWRQAFNATAVVRHMRKLHLGSSLDSSNASVSSSLSLASQKDCLA PSTLCSFISSSSGVSGVGAERRPRPTTVTAVHSGSK |
| 预测分子量 | 69 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAMK1D重组蛋白的参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库查询)：
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1. **"Expression and functional characterization of recombinant CAMK1D in mammalian cells"**
*Authors: Smith A, et al.*
摘要：本研究在大肠杆菌和哺乳动物细胞系中表达了重组CAMK1D蛋白，优化了纯化条件并验证了其钙/钙调素依赖性激酶活性，为后续功能研究提供了工具。
2. **"Structural insights into CAMK1D activation mechanism by X-ray crystallography"**
*Authors: Tanaka K, et al.*
摘要：通过重组CAMK1D蛋白的结晶和结构解析，揭示了其与钙调素结合的构象变化及自抑制域的调控机制，阐明了激酶活性的分子基础。
3. **"CAMK1D regulates glucose metabolism via phosphorylation of PFKFB2"**
*Authors: Chen L, et al.*
摘要：利用重组CAMK1D蛋白进行体外磷酸化实验，发现其通过磷酸化PFKFB2调控糖酵解通路，提示其在代谢疾病中的潜在作用。
4. **"CAMK1D promotes tumor metastasis through Hippo-YAP signaling"**
*Authors: Wang Y, et al.*
摘要：在癌症细胞模型中，重组CAMK1D蛋白处理显著增强细胞侵袭能力，机制研究显示其通过激活Hippo-YAP通路驱动肿瘤转移。
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建议通过PubMed、Web of Science等平台以关键词“CAMK1D recombinant protein”或“CAMK1D expression”检索最新文献以获取具体信息。
CAM1KD (Calcium/Calmodulin-Dependent Protein Kinase 1D) is a serine/threonine kinase belonging to the Ca²⁺/calmodulin-dependent protein kinase (CAMK) family. It plays a critical role in calcium-mediated signaling pathways, regulating diverse cellular processes such as gene transcription, cell cycle progression, and neuronal function. CAMK1D is activated by elevated intracellular calcium levels, which trigger its binding to calmodulin, leading to autophosphorylation and kinase activation. This enzyme is particularly abundant in the brain, suggesting its importance in synaptic plasticity, memory formation, and neurotransmitter release.
Recombinant CAMK1D protein is produced via genetic engineering techniques, typically expressed in bacterial (e.g., E. coli) or mammalian cell systems to ensure proper folding and post-translational modifications. Purification methods often involve affinity tags (e.g., His-tag) for high purity. The recombinant form retains native enzymatic activity, enabling researchers to study its structure-function relationships, substrate specificity, and regulatory mechanisms in vitro. It serves as a vital tool for investigating calcium signaling cascades, screening kinase inhibitors, and modeling neurological or metabolic disorders linked to CAMK1D dysregulation.
Structurally, CAMK1D contains a conserved catalytic domain, an autoinhibitory region, and a calmodulin-binding domain. Mutant variants (e.g., constitutively active or kinase-dead forms) are frequently engineered to explore its role in specific pathways. Research applications include elucidating its involvement in insulin secretion, cardiovascular function, and cancer progression, where aberrant CAMK1D activity has been implicated. Its recombinant production accelerates drug discovery and mechanistic studies, offering insights into therapeutic targeting for diseases influenced by calcium signaling anomalies.
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