| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CREB3L4 |
| Uniprot No | Q8TEY5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-395aa |
| 氨基酸序列 | MDLGIPDLLDAWLEPPEDIFSTGSVLELGLHCPPPEVPVTRLQEQGLQGWKSGGDRGCGLQESEPEDFLKLFIDPNEVYCSEASPGSDSGISEDPCHPDSPPAPRATSSPMLYEVVYEAGALERMQGETGPNVGLISIQLDQWSPAFMVPDSCMVSELPFDAHAHILPRAGTVAPVPCTTLLPCQTLFLTDEEKRLLGQEGVSLPSHLPLTKAEERVLKKVRRKIRNKQSAQDSRRRKKEYIDGLESRVAACSAQNQELQKKVQELERHNISLVAQLRQLQTLIAQTSNKAAQTSTCVLILLFSLALIILPSFSPFQSRPEAGSEDYQPHGVTSRNILTHKDVTENLETQVVESRLREPPGAKDANGSTRTLLEKMGGKPRPSGRIRSVLHADEM |
| 分子量 | 69.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**：**"Recombinant human CREB3L4 regulates ER stress response and protein secretion"**
**作者**：Li Y, et al.
**摘要**：该研究成功在大肠杆菌中表达并纯化了重组人CREB3L4蛋白，发现其通过结合内质网应激元件(ERSE)调控下游靶基因，促进分泌蛋白的加工及内质网应激适应性反应。
2. **文献名称**：**"Structural characterization of recombinant CREB3L4 and its role in cancer cell proliferation"**
**作者**：Zhang R, et al.
**摘要**：利用X射线晶体学解析重组人CREB3L4的DNA结合域结构，并通过功能实验证实其通过激活特定促癌基因(如MMP9)促进乳腺癌细胞侵袭和转移。
3. **文献名称**：**"CREB3L4 as a novel regulator of lipid metabolism: Insights from recombinant protein studies"**
**作者**：Tanaka K, et al.
**摘要**：通过重组人CREB3L4蛋白的体外实验，揭示其与脂代谢相关转录因子(如SREBP1)相互作用，调控脂肪生成相关基因表达，提示其在代谢综合征中的潜在作用。
4. **文献名称**：**"High-yield production and functional analysis of recombinant CREB3L4 in mammalian cell systems"**
**作者**：Wang H, et al.
**摘要**：报道了在哺乳动物细胞(HEK293)中高效表达重组人CREB3L4的方法，并利用ChIP-seq技术证明其广泛参与染色质重塑复合物(如SWI/SNF)的功能调控网络。
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**注释**：以上文献为示例性质，实际引用需根据真实研究调整。推荐使用PubMed、Web of Science等平台以关键词“recombinant human CREB3L4”或“CREB3L4 function”检索最新文献。
Recombinant human CREB3L4 (cAMP responsive element binding protein 3-like 4) is a transcription factor belonging to the CREB/ATF family. It shares structural homology with other CREB3 subfamily members, characterized by a basic leucine zipper (bZIP) domain for DNA binding and dimerization. Unlike its paralogs, CREB3L4 is primarily expressed in secretory tissues such as the prostate, pancreas, and gastrointestinal tract, where it localizes to the endoplasmic reticulum (ER) or Golgi apparatus under normal conditions.
Functionally, CREB3L4 is activated through regulated intramembrane proteolysis (RIP) in response to cellular stress or specific signals. Cleavage releases its N-terminal domain, which translocates to the nucleus to regulate genes involved in protein secretion, ER stress responses, and cellular proliferation. Studies link CREB3L4 to cancer progression, particularly hormone-sensitive cancers (e.g., prostate and breast), where its overexpression correlates with tumor aggressiveness and therapy resistance.
Recombinant CREB3L4 protein enables in vitro studies of its molecular interactions, post-translational modifications, and regulatory mechanisms. Its production via bacterial or mammalian expression systems facilitates structural analyses and screens for inhibitors targeting its oncogenic activity. Current research focuses on deciphering its role in Golgi stress signaling pathways and its potential as a therapeutic biomarker.
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