| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRCP |
| Uniprot No | O75575 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-148aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMEVKDANSALLSNYEVFQLLTDLKEQRKESGKNKHSSGQQNLNTITYETLKYISKTPCRHQSPEIVREFLTALKSHKLTKAEKLQLLNHRPVTAVEIQLMVEESEERLTEEQIEALLHTVTSILPAEPEAEQKKNTNSNVAMDEEDPA |
| 分子量 | 19 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CRCP蛋白的参考文献示例(注：以下内容为假设性示例，仅供参考格式和内容框架)：
---
1. **文献名称**: "Functional Characterization of Recombinant Human CRCP in β-catenin Signaling"
**作者**: Lee, S. et al.
**摘要**: 本研究成功表达并纯化了重组人CRCP蛋白，并通过体外实验证实其作为RAMP1伴侣蛋白，在调控β-catenin信号通路中起关键作用，揭示了其在细胞增殖中的分子机制。
2. **文献名称**: "High-Yield Expression and Purification of Recombinant Human CRCP in E. coli"
**作者**: Tanaka, M. et al.
**摘要**: 该文献报道了一种优化的大肠杆菌表达系统，用于高效生产可溶性的重组人CRCP蛋白，并通过质谱和圆二色光谱验证了其结构完整性，为后续功能研究提供可靠工具。
3. **文献名称**: "CRCP Interacts with GPCRs to Modulate Insulin Secretion: Insights from Recombinant Protein Studies"
**作者**: Gupta, R. et al.
**摘要**: 利用重组人CRCP蛋白进行体外结合实验，发现其与特定G蛋白偶联受体(GPCRs)相互作用，可能影响胰岛素分泌通路，为代谢疾病治疗提供新靶点。
4. **文献名称**: "CRCP as a Biomarker in Colorectal Cancer: Role of Recombinant Protein-Based Assays"
**作者**: Chen, X. et al.
**摘要**: 通过重组CRCP蛋白开发了ELISA检测方法，临床样本分析显示CRCP在结直肠癌患者中表达上调，提示其作为潜在诊断标志物的可能性。
---
**注**：上述文献为模拟示例，实际引用需以真实出版物为准。建议通过学术数据库(如PubMed、Web of Science)以关键词“recombinant human CRCP”或“CRCP protein function”检索最新研究。
**Background of Recombinant Human CRCP Protein**
Recombinant human CRCP (Cellular Retinol Binding Protein 2. also known as RBP2) is a protein encoded by the *RBP2* gene, produced using recombinant DNA technology to ensure high purity and biological activity. CRCP belongs to the retinol-binding protein family, primarily involved in retinol (vitamin A) transport and metabolism, facilitating its storage, mobilization, and signaling in cells. It plays a role in regulating cellular differentiation, immune responses, and vision-related processes.
CRCP gained attention for its interaction with G-protein-coupled receptors (GPCRs), particularly with receptor activity-modifying proteins (RAMPs), influencing receptor ligand specificity and signaling pathways. Studies suggest its involvement in cancer progression, metabolic disorders, and cardiovascular diseases, highlighting its therapeutic potential.
Recombinant CRCP is widely used in structural studies, ligand-receptor interaction assays, and drug discovery. Its production in systems like *E. coli* or mammalian cells enables scalable applications in biomedical research, aiding the exploration of CRCP's pathophysiological roles and its utility as a target for modulating GPCR-related pathways.
×
