| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CPXM1 |
| Uniprot No | Q96SM3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-734aa |
| 氨基酸序列 | MWGLLLALAAFAPAVGPALGAPRNSVLGLAQPGTTKVPGLTPALHSSPAQPPAETANGTSEQHVRIRVIKKKKVIMKKRKKLTLTRPTPLVTAGPLVTPTPAGTLDPAEKQETGCPPLGLESLRVSDSRLEASSSQSFGLGPHRGRLNIQSGLEDGDLYDGAWCAEEQDADPWFQVDAGHPTRFSGVITQGRNSVWRYDWVTSYKVQFSNDSRTWWGSRNHSSGMDAVFPANSDPETPVLNLLPEPQVARFIRLLPQTWLQGGAPCLRAEILACPVSDPNDLFLEAPASGSSDPLDFQHHNYKAMRKLMKQVQEQCPNITRIYSIGKSYQGLKLYVMEMSDKPGEHELGEPEVRYVAGMHGNEALGRELLLLLMQFLCHEFLRGNPRVTRLLSEMRIHLLPSMNPDGYEIAYHRGSELVGWAEGRWNNQSIDLNHNFADLNTPLWEAQDDGKVPHIVPNHHLPLPTYYTLPNATVAPETRAVIKWMKRIPFVLSANLHGGELVVSYPFDMTRTPWAARELTPTPDDAVFRWLSTVYAGSNLAMQDTSRRPCHSQDFSVHGNIINGADWHTVPGSMNDFSYLHTNCFEVTVELSCDKFPHENELPQEWENNKDALLTYLEQVRMGIAGVVRDKDTELGIADAVIAVDGINHDVTTAWGGDYWRLLTPGDYMVTASAEGYHSVTRNCRVTFEEGPFPCNFVLTKTPKQRLRELLAAGAKVPPDLRRRLERLRGQKD |
| 分子量 | 108.1 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人CPXM1蛋白的参考文献概括(注：CPXM1相关研究较少，部分文献为假设性示例，实际检索需结合具体数据库)：
1. **《Expression and purification of recombinant human CPXM1 for functional studies》**
*作者：Li X et al.*
摘要：研究描述了人源CPXM1蛋白在大肠杆菌中的重组表达及纯化方法，并通过体外实验表明其可能参与细胞外基质的相互作用。
2. **《Structural characterization of CPXM1 reveals its potential role in cell adhesion》**
*作者：Wang Y et al.*
摘要：通过X射线晶体学解析了重组CPXM1蛋白的3D结构，揭示其含有一个保守的金属羧肽酶结构域，并推测其可能通过整合素通路调控细胞黏附。
3. **《CPXM1 as a novel biomarker in cancer progression: Insights from recombinant protein-based assays》**
*作者：Chen H et al.*
摘要：利用重组CPXM1蛋白开发了ELISA检测方法，发现其在多种肿瘤组织中的表达水平升高，提示其可能作为癌症诊断的潜在标志物。
**提示**：实际研究中，CPXM1功能尚不明确，上述文献内容为示例性质。建议通过PubMed或Google Scholar以“recombinant CPXM1”或“CPXM1 protein function”为关键词检索最新文献。
**Background of Recomcombinant Human CPXM1 Protein**
Carboxypeptidase X Member 1 (CPXM1) is a metallocarboxypeptidase (CP) belonging to the CP subfamily, characterized by a conserved catalytic domain but lacking canonical protease activity. Unlike traditional CPs involved in peptide processing, CPXM1 is proposed to function in cell adhesion and signaling due to its unique thrombospondin type 1 (TSP1) domain, which may mediate extracellular matrix interactions. The protein is encoded by the *CPXM1* gene, located on human chromosome 20. and is expressed in tissues like the brain, immune cells, and certain cancers.
Recombinant human CPXM1 protein is engineered using expression systems (e.g., mammalian or insect cells) to produce soluble, purified forms for functional studies. Research highlights its emerging roles in cancer progression, neuronal development, and immune regulation. For instance, CPXM1 may influence tumor invasiveness via matrix metalloproteinase interactions or modulate neuronal circuit formation through adhesion pathways. Its TSP1 domain also suggests potential involvement in angiogenesis or inflammation. However, precise mechanisms remain unclear, necessitating further structural and functional exploration. Recombinant CPXM1 serves as a critical tool for elucidating these pathways and assessing its therapeutic or diagnostic potential in diseases.
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