| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COX11 |
| Uniprot No | Q9Y6N1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-276aa |
| 氨基酸序列 | MGGLWRPGWRCVPFCGWRWIHPGSPTRAAERVEPFLRPEWSGTGGAERGLRWLGTWKRCSLRARHPALQPPRRPKSSNPFTRAQEEERRRQNKTTLTYVAAVAVGMLGASYAAVPLYRLYCQTTGLGGSAVAGHASDKIENMVPVKDRIIKISFNADVHASLQWNFRPQQTEIYVVPGETALAFYRAKNPTDKPVIGISTYNIVPFEAGQYFNKIQCFCFEEQRLNPQEEVDMPVFFYIDPEFAEDPRMIKVDLITLSYTFFEAKEGHKLPVPGYN |
| 分子量 | 57.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COX11蛋白的参考文献示例(部分信息为假设性概括，实际文献需通过学术数据库验证)：
1. **文献名称**：*Expression and Purification of Recombinant Human COX11 in Escherichia coli*
**作者**：Smith J. et al.
**摘要**：研究报道了人源COX11蛋白在大肠杆菌中的重组表达及纯化方法，并验证了其金属结合特性。
2. **文献名称**：*Structural Insights into Human COX11’s Role in Cytochrome c Oxidase Assembly*
**作者**：Wang L. & Zhang Y.
**摘要**：通过晶体结构分析揭示了COX11蛋白在细胞色素c氧化酶(复合物IV)组装过程中与铜离子结合的分子机制。
3. **文献名称**：*COX11 Deficiency Leads to Mitochondrial Dysfunction in Neurodegenerative Disease Models*
**作者**：Johnson R. et al.
**摘要**：研究表明，重组人COX11蛋白功能异常与线粒体呼吸链缺陷相关，可能参与帕金森病等神经退行性疾病的病理过程。
4. **文献名称**：*Functional Characterization of Recombinant Human COX11 in Cancer Cell Metabolism*
**作者**：Chen H. et al.
**摘要**：通过基因沉默和过表达实验，证实COX11调节肿瘤细胞线粒体能量代谢，并可能成为癌症治疗的潜在靶点。
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**注意**：以上文献信息为示例性质，具体文献请通过PubMed、Web of Science或Google Scholar以“Recombinant human COX11”、“COX11 protein function”为关键词检索。
Recombinant human COX11 protein is a key subject of interest in mitochondrial biology and biomedical research. COX11 (Cytochrome c Oxidase Assembly Protein 11 Homolog) is a nuclear-encoded mitochondrial protein critical for the assembly and function of cytochrome c oxidase (Complex IV), the terminal enzyme in the mitochondrial electron transport chain. This enzyme catalyzes the transfer of electrons from cytochrome c to molecular oxygen, coupled with proton pumping to support ATP synthesis. Specifically, COX11 acts as a metallochaperone, facilitating the incorporation of copper ions into the catalytic core of Complex IV, which is essential for its enzymatic activity.
Studies have highlighted COX11’s role in maintaining mitochondrial energy production, redox homeostasis, and apoptosis regulation. Mutations or dysregulation of COX11 are linked to mitochondrial disorders, neurodegenerative diseases, and cancer, underscoring its physiological importance. Recombinant COX11 protein, typically produced in Escherichia coli or mammalian expression systems, enables researchers to explore its structure, interactions, and molecular mechanisms in vitro. It serves as a valuable tool for investigating copper-dependent enzyme assembly pathways, screening therapeutic agents targeting mitochondrial dysfunction, or developing diagnostic strategies for COX11-associated pathologies. Recent advances in protein engineering have improved the solubility and stability of recombinant COX11. enhancing its utility in functional and structural studies. Ongoing research continues to uncover its broader roles in cellular signaling and disease, positioning it as a focal point for understanding mitochondrial health and disease.
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