关键信息
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基因名
EIF2aK1
- 应用
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别名
EIF2aK1；DELE；KIAA0141；DAP3-binding cell death enhancer 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P19525
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表达区间
2-551aa
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氨基酸序列
AGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGTLEQWIEKRRGEKLDKVLALELFEQITKGVDYIHSKKLIHRDLKPSNIFLVDTKQVKIGDFGLVTSLKNDGKRTRSKGTLRYMSPEQISSQDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFDKKEKTLLQKLLSKKPEDRPNTSEILRTLTVWKKSPEKNERHTC
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分子量
64.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
EIF2AK1, also known as PKR (protein kinase R), is a crucial regulator of the cellular stress response, primarily involved in the detection of viral infections and the regulation of protein synthesis. This kinase plays a vital role in the phosphorylation of eukaryotic initiation factor 2 alpha (eIF2α), a key step that inhibits global protein translation during stress conditions such as viral pathogen invasion or nutrient deprivation. The dysregulation of EIF2AK1 has been linked to various diseases, including cancer and neurodegenerative disorders, making it an attractive target for therapeutic interventions. Recent research has focused on the structural and functional characterization of recombinant EIF2AK1 proteins, which serve as valuable tools for understanding its mechanistic roles and regulatory pathways. By producing recombinant EIF2AK1, researchers aim to elucidate how this kinase is activated in response to different stressors, investigate its interactions with other cellular proteins, and explore its potential as a biomarker for disease states or a target for drug development. Enhanced understanding of EIF2AK1 could pave the way for innovative therapies that modulate its activity to restore normal cellular functions in diseases associated with stress responses.
