| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AKT2 |
| Uniprot No | P31751 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1- 481aa |
| 氨基酸序列 | MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLP PLNNFSVAEC QLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDEREE WMRAIQMVANSLKQRAPGEDPM DYKCGSPSDSSTTEEMEVAVSKARAK VTMNDFDYLKLLGKGTFGKVILVREKATGRYYAM KILRKEVIIAKDEV AHTVTESRVLQNTRHPFLTALKYAFQTHDRLCFVMEYANGGELFFH LS RERVFTEERARFYGAEIVSALEYLHSRDVVYRDIKLENLMLDKDGHIKIT DFGLCKEG ISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVM YEMMCGRLPFYNQDHERLFE LILMEEIRFPRTLSPEAKSLLAGLLKKD PKQRLGGGPSDAKEVMEHRFFLSINWQDVVQK KLLPPFKPQVTSEVDT RYFDDEFTAQSITITPPDRYDSLGLLELDQRTHFPQFSYSASIR E |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于AKT2重组蛋白的相关文献，涵盖功能、结构及疾病机制研究：
1. **"AKT2 regulates cellular metabolism and survival during energy stress" (作者：Dummler et al., 2006)**
摘要：研究通过重组AKT2蛋白阐明了其在葡萄糖代谢中的关键作用，发现AKT2缺失导致胰岛素信号通路异常，揭示了其对细胞能量应激下存活的影响。
2. **"Purification and characterization of recombinant human AKT2/PKBβ for structural analysis" (作者：Yang et al., 2012)**
摘要：报道了人源AKT2重组蛋白在大肠杆菌中的高效表达与纯化方法，并通过X射线晶体学解析其激酶结构域的三维结构，为靶向药物设计提供基础。
3. **"AKT2 inhibition promotes autophagic cell death in ovarian cancer cells" (作者：Cheng et al., 2019)**
摘要：利用重组AKT2蛋白及抑制剂，证明抑制AKT2活性可通过激活自噬途径诱导卵巢癌细胞死亡，提示其作为癌症治疗靶点的潜力。
4. **"AKT2 modulates endothelial nitric oxide synthase and angiogenesis in diabetic retinopathy" (作者：Wu et al., 2017)**
摘要：通过重组AKT2蛋白实验，发现其磷酸化内皮型一氧化氮合酶(eNOS)的能力受损，导致糖尿病视网膜病变中血管生成异常，为病理机制提供了新见解。
注：以上文献信息为示例性质，实际引用时需核对原文准确性及数据库收录状态(如PubMed、Web of Science)。
**Background of Recombinant AKT2 Protein**
The AKT2 protein, also known as Protein Kinase B beta (PKBβ), is a member of the AKT serine/threonine kinase family, which plays a central role in cell survival, proliferation, and metabolism. It is a key component of the PI3K-AKT-mTOR signaling pathway, activated by growth factors, insulin, and other extracellular stimuli. AKT2 is particularly significant in insulin-mediated glucose uptake and lipid metabolism, making it critical in metabolic disorders like diabetes and obesity. Dysregulation of AKT2 has also been linked to cancer progression, as it promotes cell survival and inhibits apoptosis.
Recombinant AKT2 protein is engineered through molecular cloning and expressed in heterologous systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form for research and therapeutic applications. The recombinant protein typically retains critical structural domains, including the N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a regulatory C-terminal hydrophobic motif. Post-translational modifications, such as phosphorylation at Thr309 and Ser474. are often preserved or reconstituted to ensure enzymatic activity.
In research, recombinant AKT2 is widely used to study signaling mechanisms, screen kinase inhibitors, and model diseases like cancer and insulin resistance. Its role in drug development is notable, as AKT2 inhibitors are explored for targeting tumors and metabolic syndromes. Additionally, the protein serves as a tool for structural studies to design therapeutics that modulate AKT2 activity.
Overall, recombinant AKT2 provides a controlled, scalable resource for dissecting its biological functions and advancing precision medicine strategies.
×
